Yin L G, Zou Z Q, Zhao H Y, Zhang C L, Shen J G, Qi L, Qi M, Xue Z Q
Mol Biol (Mosk). 2014 Sep-Oct;48(5):797-804. doi: 10.7868/s0026898414050188.
Adenocarcinoma (ADC) and squamous cell carcinomas (SCC) are two subtypes of non-small cell lung carcinomas which are regarded as the leading cause of cancer-related malignancy worldwide. The aim of this study is to detect the differentially methylated loci (DMLs) and differentially methylated genes (DMGs) of these two tumor sets, and then to illustrate the different expression level of specific methylated genes. Using TCGA database and Illumina HumanMethylation 27 arrays, we first screened the DMGs and DMLs in tumor samples. Then, we explored the BiologicalProcess terms of hypermethylated and hypomethylated genes using Functional Gene Ontology (GO) catalogues. Hypermethylation intensively occurred in CpG-island, whereas hypomethylation was located in non-CpG-island. Most SCC and ADC hypermethylated genes involved GO function of DNA dependenit regulation of transcription, and hypomethylated genes mainly 'enriched in the term of immune responses. Additionally, the expression level of specific differentially methylated genesis distinctbetween ADC and SCC. It is concluded that ADC and SCC have different methylated status that might play an important role in carcinogenesis.
腺癌（ADC）和鳞状细胞癌（SCC）是非小细胞肺癌的两种亚型，被认为是全球癌症相关恶性肿瘤的主要原因。本研究的目的是检测这两种肿瘤类型的差异甲基化位点（DMLs）和差异甲基化基因（DMGs），然后阐明特定甲基化基因的不同表达水平。利用TCGA数据库和Illumina HumanMethylation 27芯片，我们首先在肿瘤样本中筛选出DMGs和DMLs。然后，我们使用功能基因本体（GO）目录探索高甲基化和低甲基化基因的生物学过程术语。高甲基化主要发生在CpG岛，而低甲基化位于非CpG岛。大多数SCC和ADC高甲基化基因涉及DNA依赖性转录调控的GO功能，低甲基化基因主要富集在免疫反应方面。此外，特定差异甲基化基因的表达水平在ADC和SCC之间存在差异。结论是，ADC和SCC具有不同的甲基化状态，这可能在致癌过程中起重要作用。
