Meineke I, Stüwe E, Henne E M, Rusteberg G, Brendel E, De Mey C
SK&F Institute for Applied Clinical Pharmacology, Göttingen, F.R.G.
J Chromatogr. 1989 Sep 1;493(2):287-303. doi: 10.1016/s0378-4347(00)82735-8.
A method for the simultaneous determination of epinephrine, norepinephrine and dopamine in human plasma is described, which combines the advantages of liquid-liquid extraction sample preparation, high-performance liquid chromatography on weak cation-exchange stationary phases and dual-electrode coulometric detection. The limits of quantification are less than 5 pg/ml (at a signal-to-noise ratio greater than 5) for each analyte. The influence of various experimental parameters (e.g., composition of the mobile phase, pretreatment of the assay buffer, components of the re-extraction system) on the performance of the assay is reported in detail. A number of applications are presented, which demonstrate the quality of the data obtained in terms of sensitivity, reproducibility and significance.
