Raggi M A, Sabbioni C, Casamenti G, Gerra G, Calonghi N, Masotti L
Department of Pharmaceutical Sciences, University of Bologna, Italy.
J Chromatogr B Biomed Sci Appl. 1999 Jul 9;730(2):201-11. doi: 10.1016/s0378-4347(99)00213-3.
In the present study, assays were improved for the determination of catecholamines in human plasma. High-performance liquid chromatography with electrochemical detection was employed for quantitative analysis. The influence of various parameters on chromatographic performance, such as the composition and the pH of the mobile phase, and the detection potential, was investigated. An accurate solid-phase extraction procedure, after catecholamine complexation with diphenylborate, was developed. The efficiency yield for all catecholamines was in the range 92-98%. Relative standard deviation values for repeatability and for intermediate precision were less than 2% and 3%, respectively, for all three analytes.
在本研究中，对人血浆中儿茶酚胺的测定方法进行了改进。采用高效液相色谱-电化学检测法进行定量分析。研究了各种参数对色谱性能的影响，如流动相的组成和pH值以及检测电位。在儿茶酚胺与二苯基硼酸盐络合后，开发了一种准确的固相萃取方法。所有儿茶酚胺的提取效率在92%-98%之间。所有三种分析物的重复性相对标准偏差值和中间精密度相对标准偏差值分别小于2%和3%。
