Lomakina A V, Pogodaeva T V, Morozov I V, Zemskaya T I
Mikrobiologiia. 2014 May-Jun;83(3):355-65.
At the site of natural ingress of oil microbial diversity in the Central Baikal bottom sediments differing in the chemical composition of pore waters was studied by molecular biological techniques. The sediments saturated with oil and methane were found to contain members of 10 bacterial and 2 archaeal phyla. The oxidized sediment layer contained methanotrophic bacteria belonging to the Alphaproteobacteria, which had a specific structure of the pmoA gene and clustered together with uncultured methanotrophs from cold ecosystems. The upper sediment layer contained also oil-oxidizing bacteria and the alkB genes most colsely related to those of Rhodococcus. The microbial community of reduced sediments exhibited lower diversity and was represented mostly by the organisms involved in hydrocarbon biodegradation.
利用分子生物学技术，研究了贝加尔湖中部底部沉积物中石油自然进入位点处孔隙水化学成分不同的微生物多样性。发现富含石油和甲烷的沉积物包含10个细菌门和2个古菌门的成员。氧化沉积层含有属于α-变形菌纲的甲烷氧化细菌，其pmoA基因具有特定结构，并与来自寒冷生态系统的未培养甲烷氧化菌聚集在一起。上部沉积层还含有石油氧化细菌以及与红球菌的alkB基因关系最密切的基因。还原沉积物的微生物群落多样性较低，主要由参与碳氢化合物生物降解的生物组成。
