Bruno Barbara, Maurella Cristiana, Falco Sara, Tarducci Alberto, Zanatta Renato, Gianella Paola, D'Angelo Antonio, Piras Lisa, Di Bella Andrea, Borrelli Antonio
Department of Animal Pathology, Clinical Medicine Section, Grugliasco, Torino, Italy.
the Istituto Zooprofilattico Sperimentale del Piemonte, Torino, Italy.
J Vet Emerg Crit Care (San Antonio). 2015 May-Jun;25(3):358-63. doi: 10.1111/vec.12300. Epub 2015 Apr 2.
To evaluate blood coagulation using thromboelastometry in dogs following orthopedic surgery.
Longitudinal observational study.
University veterinary teaching hospital.
Thirty-four adult client-owned dogs.
Dogs undergoing elective or emergency orthopedic surgery had whole blood collected before (T0), at 24 hours (T1), and 1 week (T2) after surgery. Whole blood from each dog was collected by jugular venipuncture using a 20-Ga needle and minimum venous stasis. The blood was placed into tubes containing 3.8% trisodium citrate (1 part citrate: 9 parts blood) and rested at 37°C. Coagulation was evaluated by means of thromboelastometry using the in-TEM, ex-TEM, and fib-TEM assays. Statistically significant increases (P < 0.05) in maximum clot firmness (MCF) from T0 to T1 in the in-TEM and fib-TEM profiles (both P = 0.0001), from T0 to T2 in the in-TEM, ex-TEM, and fib-TEM profiles (P = 0.012, P = 0.037, and P = 0.0001, respectively), and from T1 to T2 in the fib-TEM profile (P = 0.039) were noted. The α angle increased from T0 to T2 in the in-TEM and ex-TEM profiles (P = 0.019 and P = 0.036, respectively). All results were, however, within the institutional reference ranges.
In this study, unlike the hypercoagulability observed in human orthopedic patients, a hypercoagulable state as measured by thromboelastometry did not develop in dogs following orthopedic surgery.
采用血栓弹力图评估骨科手术后犬的凝血情况。
纵向观察性研究。
大学兽医教学医院。
34只成年客户拥有的犬。
接受择期或急诊骨科手术的犬在术前（T0）、术后24小时（T1）和术后1周（T2）采集全血。使用20G针头经颈静脉穿刺采集每只犬的全血，并尽量减少静脉淤滞。将血液置于含有3.8%柠檬酸钠（1份柠檬酸盐：9份血液）的试管中，在37°C下静置。采用血栓弹力图通过体内血栓弹力图（in-TEM）、体外血栓弹力图（ex-TEM）和纤维蛋白血栓弹力图（fib-TEM）检测评估凝血情况。体内血栓弹力图和纤维蛋白血栓弹力图检测中，从T0到T1最大血凝块硬度（MCF）有统计学显著增加（P < 0.05）（两者P = 0.0001），在体内血栓弹力图、体外血栓弹力图和纤维蛋白血栓弹力图检测中，从T0到T2最大血凝块硬度也有统计学显著增加（分别为P = 0.012、P = 0.037和P = 0.0001），在纤维蛋白血栓弹力图检测中，从T1到T2最大血凝块硬度有统计学显著增加（P = 0.039）。体内血栓弹力图和体外血栓弹力图检测中，α角从T0到T2增加（分别为P = 0.019和P = 0.036）。然而，所有结果均在机构参考范围内。
在本研究中，与人类骨科患者中观察到的高凝状态不同，采用血栓弹力图检测，犬在骨科手术后未出现高凝状态。
