Morgunov Igor G, Kamzolova Svetlana V
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow, 142290, Russia,
Appl Microbiol Biotechnol. 2015 Aug;99(15):6443-50. doi: 10.1007/s00253-015-6558-5. Epub 2015 Apr 7.
In this study, physiologo-biochemical characteristics of citrate-producing yeast Yarrowia lipolytica grown on glycerol-containing waste of biodiesel industry were studied by an investigation of growth dynamics, the consumption of glycerol, and the fatty acid fractions from waste as well as by measuring the activities of enzymes involved in the metabolism of waste. It was shown that Y. lipolytica realizes concurrent uptake of glycerol and the fatty acid fractions during conversion of glycerol-containing waste, although glycerol was utilized at a higher rate than fatty acids. Under optimal feeding of glycerol-containing waste by portions of 20 g l(-1), the citric acid production and the ratio between citric acid and isocitric acid depended on the strain used. It was revealed that wild strain Y. lipolytica VKM Y-2373 produced citrate and isocitrate with a ratio of 1.7:1, while the mutant strain Y. lipolytica NG40/UV7 synthesized presumably citric acid (122.2 g l(-1)) with a citrate-to-isocitrate ratio of 53:1 and the yield of 0.95 g g(-1).
在本研究中，通过研究生长动力学、甘油消耗、废水中脂肪酸组分以及测量参与废水代谢的酶的活性，对在生物柴油行业含甘油废料上生长的产柠檬酸酵母解脂耶氏酵母的生理生化特性进行了研究。结果表明，在含甘油废料的转化过程中，解脂耶氏酵母能够同时摄取甘油和脂肪酸组分，尽管甘油的利用速率高于脂肪酸。在以20 g l⁻¹的量分批优化投喂含甘油废料的情况下，柠檬酸产量以及柠檬酸与异柠檬酸的比例取决于所使用的菌株。结果显示，野生型解脂耶氏酵母VKM Y - 2373产生的柠檬酸和异柠檬酸的比例为1.7:1，而突变型解脂耶氏酵母NG40/UV7可能合成了柠檬酸（122.2 g l⁻¹），柠檬酸与异柠檬酸的比例为53:1，产率为0.95 g g⁻¹。
