Kinoshita Yuta, Niwa Hidekazu, Katayama Yoshinari
Microbiology Division, Epizootic Research Center, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Shimotuke, Tochigi, 329-0412, Japan.
Microbiol Immunol. 2015 Jun;59(6):365-70. doi: 10.1111/1348-0421.12257.
Microbial substitution occasionally occurs following the administration of antimicrobials to horses that have pneumonia or pleuropneumonia. Four specific loop-mediated isothermal amplification (LAMP) assays were developed to detect some equine respiratory pathogens, namely strains of the Bacteroides-Prevotella group, Klebsiella pneumoniae, Stenotrophomonas maltophilia, and Staphylococcus aureus. These four LAMP assays and two previously published LAMP assays targeting Escherichia coli or Pseudomonas aeruginosa were used on clinical respiratory specimens and a high accordance found between the results of the LAMP assays and bacterial culture. Use of these LAMP assays could enable rapid detection of pathogenic bacteria and swift administration of the appropriate antimicrobials.
对患有肺炎或胸膜肺炎的马匹使用抗菌药物后偶尔会发生微生物替代现象。开发了四种特异性环介导等温扩增（LAMP）检测方法，以检测一些马呼吸道病原体，即拟杆菌-普雷沃菌属菌株、肺炎克雷伯菌、嗜麦芽窄食单胞菌和金黄色葡萄球菌。将这四种LAMP检测方法以及之前发表的两种针对大肠杆菌或铜绿假单胞菌的LAMP检测方法应用于临床呼吸道标本，发现LAMP检测结果与细菌培养结果高度一致。使用这些LAMP检测方法能够快速检测病原菌并迅速给予适当的抗菌药物。
