Ohashi Y, Takeshita T, Nagata K, Mori S, Sugamura K
Department of Microbiology, Tohoku University School of Medicine, Sendai, Japan.
J Immunol. 1989 Dec 1;143(11):3548-55.
Unstimulated PBL were examined for expression of IL-2R subunits, IL-2Rp55 and IL-2Rp75, by two-color flow cytometric analyses using mAb. NKH-1+ non-T non-B cells expressed IL-2Rp75 but not IL-2Rp55, and the IL-2Rp75 sites on purified NKH-1+ cells were determined to be 1630 sites/cell by binding of 125I-labeled TU27 mAb specific for IL-2Rp75. In the CD4+ T cell population, IL-2Rp55+ cells were significantly detected, but little or marginally of the IL-2Rp75+ cells. However, IL-2Rp75+ cells were significantly detected, but little of the IL-2Rp55+ cells in the CD8+ T cell population. The IL-2Rp75 sites on CD8+ T cells were estimated at approximate 180-410 sites/cell. In the CD4+ T cells, expression of IL-2Rp75 as well as IL-2Rp55 was induced by stimulation with PHA. IL-2Rp75+ cells, but not IL-2Rp55+ cells, were also detected in the CD14+ monocyte population. In the CD20+ B cell population, a small number of IL-2Rp55+ cells was detected, but little of the IL-2Rp75+ cells.
使用单克隆抗体通过双色流式细胞术分析未刺激的外周血淋巴细胞（PBL）中白细胞介素-2受体（IL-2R）亚基IL-2Rp55和IL-2Rp75的表达。NKH-1 +非T非B细胞表达IL-2Rp75但不表达IL-2Rp55，通过与IL-2Rp75特异性的125I标记的TU27单克隆抗体结合，确定纯化的NKH-1 +细胞上的IL-2Rp75位点为1630个位点/细胞。在CD4 + T细胞群体中，可显著检测到IL-2Rp55 +细胞，但IL-2Rp75 +细胞很少或仅有少量。然而，在CD8 + T细胞群体中可显著检测到IL-2Rp75 +细胞，但IL-2Rp55 +细胞很少。CD8 + T细胞上的IL-2Rp75位点估计约为180 - 410个位点/细胞。在CD4 + T细胞中，用PHA刺激可诱导IL-2Rp75以及IL-2Rp55的表达。在CD14 +单核细胞群体中也检测到IL-2Rp75 +细胞，但未检测到IL-2Rp55 +细胞。在CD20 + B细胞群体中，检测到少量IL-2Rp55 +细胞，但IL-2Rp75 +细胞很少。
