白细胞介素-2 受体β链作为低剂量吗氟苯哒嗪的可能靶点。
Interleukin-2 receptor beta chain as a possible target for low doses of mafosfamide.
机构信息
Research Centre for Medical Genetics Moskvorechie St. Moscow 115478 Russia.
出版信息
Mediators Inflamm. 1995;4(3):175-80. doi: 10.1155/S0962935195000287.
The 7-day cytotoxic lymphocytes (CTL) induced in mixed lymphocyte culture express only the chain of the interleukin-2 receptor (IL-2R). In the present study this fact has been confirmed in a murine semi-allogeneic system. The ability of low doses of mafosfamide (Mf) to affect IL-2-induced CTL proliferation has been demonstrated. It was also shown that IL-2 activated resting suppressor cells. The pretreatment of the suppressor cells with either monoclonal antibodies (mAbs) against the p75 chain of IL-2R, or with Mf abolished the suppressive effect of these cells. No restoration of the proliferative response occurred when the anti-IL-2Ralpha mAb had been used. Flow cytometry analysis of 7-day CTL was carried out with mAbs against the alpha and beta chains of IL-2R. CTL treatment with Mf inhibited anti-IL-2Rbeta mAb binding. It may be assumed that the anti-proliferative effects of Mf which have been demonstrated in this paper, were a result of blocking the IL-2R beta chain.
在混合淋巴细胞培养中诱导的 7 天细胞毒性淋巴细胞 (CTL) 仅表达白细胞介素 2 受体 (IL-2R) 的链。在本研究中，在鼠半同种异体系统中证实了这一事实。已经证明低剂量的 mafosfamide (Mf) 能够影响 IL-2 诱导的 CTL 增殖。还表明 IL-2 激活了静止的抑制细胞。用针对 IL-2R 的 p75 链的单克隆抗体 (mAb) 或用 Mf 预处理抑制细胞，可消除这些细胞的抑制作用。当使用抗 IL-2Ralpha mAb 时，不会发生增殖反应的恢复。用针对 IL-2R 的 alpha 和 beta 链的 mAb 对 7 天 CTL 进行流式细胞术分析。CTL 用 Mf 处理可抑制抗 IL-2Rbeta mAb 的结合。可以假设，本文中证明的 Mf 的抗增殖作用是由于阻断了 IL-2R beta 链。
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