Plant-Microbial Interaction Research Group, Institute of Environmental Science, Jagiellonian University, Krakow, Poland.
Department of Cell Biology and Imaging, Institute of Zoology, Jagiellonian University, Krakow, Poland.
Sci Total Environ. 2015 Jul 15;521-522:305-14. doi: 10.1016/j.scitotenv.2015.03.101. Epub 2015 Apr 3.
The presence of moulds in indoor environments causes serious diseases and acute or chronic toxicological syndromes. In order to inhibit or prevent the growth of microorganisms on building materials, the disruption of their vital processes or the reduction of reproduction is required. The development of novel techniques that impair the growth of microorganisms on building materials is usually based on silver nanoparticles (AgNPs). It makes them an alternative to other biocides. AgNPs have proven antibacterial activity and became promising in relation to fungi. The aim of the study was to assess growth and morphology of mycelia of typical indoor fungal species: Penicillium brevicompactum, Aspergillus fumigatus, Cladosporium cladosporoides, Chaetomium globosum and Stachybotrys chartarum as well as Mortierella alpina, cultured on agar media. The antifungal activity of AgNPs was also tested in relation to C. globosum and S. chartarum grown on the surface of gypsum drywall. It was found that the presence of AgNPs in concentrations of 30-200mg/l significantly decreased the growth of fungi. However, in the case of M. alpina, AgNPs stimulated its growth. Moreover, strong changes in moulds morphology and colour were observed after administration of AgNPs. Parameters of conidiophores/sporangiophores varied depending on mould region and changed significantly after treatment with AgNPs. The experiments have shown antifungal properties of AgNPs against common indoor mould species. Their application to building materials could effectively protect indoor environments from mould development. However, consideration must be given to the fact that the growth of some fungal strains might be stimulated by AgNPs.
室内环境中霉菌的存在会导致严重的疾病和急性或慢性毒理学综合征。为了抑制或防止微生物在建筑材料上的生长，需要破坏其生命过程或减少繁殖。开发新的技术来破坏建筑材料上微生物的生长通常基于银纳米粒子（AgNPs）。这使它们成为其他杀菌剂的替代品。AgNPs 已被证明具有抗菌活性，并在真菌方面具有广阔的应用前景。本研究的目的是评估典型室内真菌物种（Penicillium brevicompactum、Aspergillus fumigatus、Cladosporium cladosporoides、Chaetomium globosum 和 Stachybotrys chartarum）以及Mortierella alpina 的菌丝体的生长和形态，这些真菌在琼脂培养基上培养。还测试了 AgNPs 对在石膏墙板表面生长的 C. globosum 和 S. chartarum 的抗真菌活性。结果发现，AgNPs 的浓度为 30-200mg/l 时，明显抑制了真菌的生长。然而，在 M. alpina 的情况下，AgNPs 刺激了其生长。此外，AgNPs 给药后观察到霉菌形态和颜色发生强烈变化。分生孢子梗/孢子梗的参数取决于霉菌区域，并且在用 AgNPs 处理后发生显著变化。实验表明 AgNPs 对常见室内霉菌具有抗真菌特性。将其应用于建筑材料可以有效地防止室内环境中霉菌的生长。然而，必须考虑到 AgNPs 可能会刺激某些真菌菌株的生长。
