Lane A T, Scott G A, Day K H
Department of Dermatology, University of Rochester School of Medicine and Dentistry, New York 14642.
J Invest Dermatol. 1989 Dec;93(6):787-91. doi: 10.1111/1523-1747.ep12284423.
Thirty-five human fetal skin (HFS) grafts were transplanted to nude mice for 7 to 70 d and evaluated histologically with 64 biopsies. The estimated gestational ages (EGA) of the grafts at the time of the transplantation ranged from 8 to 19 weeks. The maturation of the engrafted fetal skin was evaluated by assessing epidermal, dermal, and appendage development. Within the nude mouse, the HFS demonstrated progression in stratification and maturation of the epidermis. The dermis increased in depth, adding fibrovascular stroma and adipose tissue. The appendages demonstrated invagination, differentiation, and progression of organogenesis. Subcutaneously placed grafts showed the same rate of HFS development as HFS in utero. The grafts transplanted to the surface of the nude mice and exposed to air demonstrated an acceleration of development. We conclude that HFS transplanted to the nude mouse is an effective in vivo model for maintaining and altering HFS maturation.
将35块人胎儿皮肤（HFS）移植到裸鼠体内7至70天，并通过64次活检进行组织学评估。移植时移植物的估计胎龄（EGA）为8至19周。通过评估表皮、真皮和附属器的发育来评价植入胎儿皮肤的成熟度。在裸鼠体内，HFS显示出表皮分层和成熟的进展。真皮深度增加，添加了纤维血管基质和脂肪组织。附属器表现出内陷、分化和器官发生的进展。皮下植入的移植物显示出与子宫内HFS相同的发育速度。移植到裸鼠表面并暴露于空气中的移植物显示出发育加速。我们得出结论，移植到裸鼠体内的HFS是维持和改变HFS成熟度的一种有效的体内模型。
