Abraham W, Downing D T
Marshall Dermatology Research Laboratories, Department of Dermatology, University of Iowa, Iowa City.
J Invest Dermatol. 1989 Dec;93(6):809-13. doi: 10.1111/1523-1747.ep12284431.
Liposomes were prepared from stratum corneum lipids consisting of epidermal ceramides (55% by weight), cholesterol (25%), free fatty acids (15%), and cholesteryl sulfate (5%). Multiple lamellae were formed by air-drying the liposomal suspensions on hydrophilic filter disks, and water permeabilities through these filter-supported lamellae were measured using a diffusion cell. Ultrastructure of the lipid lamellae was characterized by scanning and thin-section electron microscopy. Water flux data and ultrastructure of the model membranes are discussed in relation to stratum corneum, the horny layer of the epidermis that constitutes the epidermal barrier.
脂质体由角质层脂质制备而成，角质层脂质包含表皮神经酰胺（重量占比55%）、胆固醇（25%）、游离脂肪酸（15%）和硫酸胆固醇（5%）。通过将脂质体悬浮液在亲水性滤盘上进行空气干燥形成多层结构，并使用扩散池测量通过这些滤膜支撑的多层结构的水渗透性。通过扫描电子显微镜和超薄切片电子显微镜对脂质多层结构的超微结构进行表征。结合构成表皮屏障之一的角质层（表皮的角质层），讨论了模型膜的水通量数据和超微结构。
