[Free thyroxine immunoassay: analytical review].

作者信息

Doggui Radhouene, Ingrand Jacques

机构信息

Laboratoire de surveillance et épidémiologie nutritionnelles en Tunisie (SURVEN), Institut national de nutrition et de technologie alimentaire, Tunis, Tunisie.

Acorata, Unité de formation continue, Sajas, France.

出版信息

Ann Biol Clin (Paris). 2015 Mar-Apr;73(2):199-211. doi: 10.1684/abc.2015.1031.

DOI:10.1684/abc.2015.1031

PMID:25847744

Abstract

Thyroid hormones assays (T4 and T3) are limited to the free forms with hormonal activity, the only ones useful for the assessment of thyroid function. Free thyroxine assays are part of prescribed parameters by the physician once known plasma TSH concentration. A reference method exists, but immunoassays are the most commonly technics used in current practice. After a reminder of the iodothyronine biochemistry and physiology, the authors discuss preanalytical and analytical steps in detail, focusing on the possible pitfalls.

摘要