Minardi Daniele, Lucarini Guendalina, Simonetti Oriana, Di Primio Roberto, Montironi Rodolfo, Muzzonigro Giovanni
Institute of Maternal and Children's Sciences-Urology, Polytechnic University of the Marche Region, Azienda Ospedaliero- Universitaria Ospedali Riuniti, Ancona.
Arch Ital Urol Androl. 2015 Mar 31;87(1):33-7. doi: 10.4081/aiua.2015.1.33.
to investigate the role of CD- 44 immunohistochemical expression within tumoural and non-tumoural tissue, aiming to understand if it can help us to predict the need of performing inguinal lymph nodes dissection to complete surgery of the penis.
CD44 immunohistochemical expression was investigated in tissue specimens from 39 patients with squamous cell carcinoma of the penis who underwent partial or total penectomy between 1987 and 2008. Patient age, tumour size, and grade; CD44 intensity score, cytological expression, topographic and distribution pattern were evaluated by immunohistochemistry on archived material and correlated with disease-specific survival.
mean patients age was 67.7 years; mean followup was 130.44 months. Bilateral inguinal lymphadenectomy was performed in 14 patients; there were 8 N+ patients (23.5%). pTis-pT1 vs. > pT1 and the EAU classification of risk group resulted to be predictive of lymph nodal metastases at univariate analysis (respectively p = 0.006 and p = 0.045), but not the grading. The intensity score, cytological expression, topographic and distribution pattern of CD44 staining did not correlate with stage, grade and lymph nodes metastases. All disease related deaths occurred only in patients showing an high CD44 intratumoral expression, but this correlation is not statistically significant. Multivariate analysis showed that only lymph node metastasis was an independent prognostic factor predictive of lymph nodes metastases.
CD44 expression in patients with squamous cell carcinoma of the penis is not able to predict the need of performing inguinal lymphadenectomy; staging and the EAU classification of risk group resulted to be predictive of lymph nodal metastases.
研究CD - 44免疫组化表达在肿瘤组织和非肿瘤组织中的作用，旨在了解其是否有助于预测阴茎手术中进行腹股沟淋巴结清扫的必要性。
对1987年至2008年间接受部分或全阴茎切除术的39例阴茎鳞状细胞癌患者的组织标本进行CD44免疫组化表达研究。通过对存档材料进行免疫组化评估患者年龄、肿瘤大小和分级；CD44强度评分、细胞学表达、拓扑学和分布模式，并与疾病特异性生存率相关联。
患者平均年龄为67.7岁；平均随访时间为130.44个月。14例患者进行了双侧腹股沟淋巴结清扫术；有8例N +患者（23.5%）。在单因素分析中，pTis - pT1与> pT1以及欧洲泌尿外科学会（EAU）风险组分类可预测淋巴结转移（分别为p = 0.006和p = 0.045），但分级不能预测。CD44染色的强度评分、细胞学表达、拓扑学和分布模式与分期、分级和淋巴结转移无关。所有与疾病相关的死亡仅发生在肿瘤内CD44高表达的患者中，但这种相关性无统计学意义。多因素分析表明，只有淋巴结转移是预测淋巴结转移的独立预后因素。
阴茎鳞状细胞癌患者中CD44表达不能预测进行腹股沟淋巴结清扫的必要性；分期和EAU风险组分类可预测淋巴结转移。
