Huang Cuiqin, You Jingzhou, Nai Fangfang
College of Life Sciences, Longyan University, Longyan, China ; Engineering Research Center for the Prevention and Control of Zoonosis, Longyan, China.
College of Life Sciences, Longyan University, Longyan, China ; Longyan Entry-Exit Inspection and Quarantine Burean of the People's Republic of China, Longyan, China.
Iran J Parasitol. 2014 Apr-Jun;9(2):209-17.
Parasitic nematodes cause animal and human diseases of major socio-economic importance worldwide. The suppression of parasite development at particular developmental stages could provide an alternative approach for nematode control. In this study, Ascaris suum was used as a model system in the study of the differentially expressed genes in the infective L3 stage.
The gene (07E12) was screened and identified from the subtractive cDNA library for the infective larvae of Ascaris suum using real-time quantitative PCR. Then, the full-length cDNA of 07E12 was characterized by 3' and 5' rapid amplification of cDNA ends (RACE). The characteristics of the gene were further analyzed using bioinformatic analyses.
The results showed that the gene 07E12 was differentially expressed in the third-stage larvae of A. suum and its expression level in the infective larvae was much higher than in other stages. It was shown that the gene 07E12 had 99% identity with the corresponding sequences of the A. suum whole genome shotgun sequence containing the homologous sequences with conserved sequences of Neuropeptide-Like Protein family member. Likewise, by performing BLASTN and BLASTP searches in the GenBank™, it was shown that this gene had 99 % identity with A. suum cre-nlp-2 protein.
This gene 07E12 which is differentially expressed in the third-stage larvae of A. suum may encode a neuropeptide-like protein family member, a very important molecule in the process of infecting a host.
寄生线虫在全球范围内引发具有重大社会经济影响的动物和人类疾病。在特定发育阶段抑制寄生虫发育可为线虫控制提供一种替代方法。在本研究中，猪蛔虫被用作研究感染性L3阶段差异表达基因的模型系统。
使用实时定量PCR从猪蛔虫感染性幼虫的消减cDNA文库中筛选并鉴定基因（07E12）。然后，通过cDNA末端的3'和5'快速扩增（RACE）对07E12的全长cDNA进行表征。使用生物信息学分析进一步分析该基因的特征。
结果表明，基因07E12在猪蛔虫第三期幼虫中差异表达，其在感染性幼虫中的表达水平远高于其他阶段。结果表明，基因07E12与猪蛔虫全基因组鸟枪序列的相应序列具有99%的同一性，该序列包含与神经肽样蛋白家族成员保守序列的同源序列。同样，通过在GenBank™中进行BLASTN和BLASTP搜索，表明该基因与猪蛔虫cre-nlp-2蛋白具有99%的同一性。
在猪蛔虫第三期幼虫中差异表达的该基因07E12可能编码一种神经肽样蛋白家族成员，这是感染宿主过程中的一个非常重要的分子。
