海葵 Kv1 钾通道毒素的筛选与 cDNA 克隆。
Screening and cDNA cloning of Kv1 potassium channel toxins in sea anemones.
机构信息
Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Konan-4, Minato-ku, Tokyo, Japan.
出版信息
Mar Drugs. 2010 Dec 2;8(12):2893-905. doi: 10.3390/md8122893.
When 21 species of sea anemones were screened for Kv1 potassium channel toxins by competitive inhibition of the binding of (125)I-α-dendrotoxin to rat synaptosomal membranes, 11 species (two species of Actiniidae, one species of Hormathiidae, five species of Stichodactylidae and three species of Thalassianthidae) were found to be positive. Furthermore, full-length cDNAs encoding type 1 potassium channel toxins from three species of Stichodactylidae and three species of Thalassianthidae were cloned by a combination of RT-PCR, 3'RACE and 5'RACE. The precursors of these six toxins are commonly composed of signal peptide, propart and mature peptide portions. As for the mature peptide (35 amino acid residues), the six toxins share more than 90% sequence identities with one another and with κ(1.3)-SHTX-She1a (Shk) from Stichodactyla helianthus but only 34-63% identities with the other type 1 potassium channel toxins.
当对 21 种海葵进行 Kv1 钾通道毒素的筛选，以竞争抑制（125）I-α-芋螺毒素与大鼠突触体膜的结合时，发现 11 种（2 种 Actiniidae、1 种 Hormathiidae、5 种 Stichodactylidae 和 3 种 Thalassianthidae）为阳性。此外，通过 RT-PCR、3'RACE 和 5'RACE 的组合，从 3 种 Stichodactylidae 和 3 种 Thalassianthidae 克隆了编码 1 型钾通道毒素的全长 cDNA。这六种毒素的前体通常由信号肽、前肽和成熟肽部分组成。对于成熟肽（35 个氨基酸残基），这六种毒素彼此之间以及与来自石斑鱼的κ(1.3)-SHTX-She1a (Shk)具有超过 90%的序列同一性，但与其他 1 型钾通道毒素只有 34-63%的同一性。
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