Ogawa K, Oguchi M, Nakashima Y, Yamabe H
Department of Pathology, Faculty of Medicine, Kyoto University, Japan.
J Neurooncol. 1989 Nov;7(4):357-66. doi: 10.1007/BF02147093.
One hundred-twenty seven human brain tumors were examined by an immunoperoxidase technique for the expression of collagen Type IV, a major constituent of basement membrane. The parenchymal components were negative for the marker protein in all tumors except for neurilemmomas which were positively stained. In every case, the antibody to collagen Type IV showed distinct staining of the vascular pattern. In gliomas, capillaries increased in number and the vascular staining increased in intensity. Fine branching capillaries and endothelial glomeruloid proliferations characteristic each of oligodendrogliomas and glioblastomas could be distinctly illustrated. In two ependymomas, marked capillary proliferation was noted in periventricular areas. Fibrillar staining was observed between the tumor cells in seven of 34 meningiomas. Pericapillary lamellar deposition of collagen Type IV suggests a vascular origin of psammoma bodies. In some malignant tumors, pial-glial membranes were disrupted and the Virchow-Robin spaces were filled with malignant cells. Collagen Type IV was absent around the stromal cells of hemangioblastomas, suggesting that these stromal cells were unrelated histogenetically with endothelial cells. Collage Type IV may be useful in the differential diagnosis between meningiomas and neurilemmomas.
采用免疫过氧化物酶技术对127例人脑肿瘤进行检测，以观察基底膜主要成分IV型胶原的表达情况。除神经鞘瘤呈阳性染色外，所有肿瘤的实质成分对标记蛋白均呈阴性。在每种情况下，IV型胶原抗体均显示出明显的血管染色模式。在胶质瘤中，毛细血管数量增加，血管染色强度增强。少突胶质细胞瘤和胶质母细胞瘤各自特有的细分支毛细血管和内皮细胞肾小球样增生均可清晰显示。在2例室管膜瘤中，脑室周围区域可见明显的毛细血管增生。在34例脑膜瘤中的7例中，肿瘤细胞之间观察到纤维状染色。IV型胶原在砂粒体周围的毛细血管层状沉积提示砂粒体起源于血管。在一些恶性肿瘤中，软脑膜-胶质膜被破坏，Virchow-Robin间隙充满恶性细胞。血管母细胞瘤的基质细胞周围不存在IV型胶原，提示这些基质细胞与内皮细胞在组织发生上无关。IV型胶原可能有助于脑膜瘤和神经鞘瘤的鉴别诊断。
