运用聚合酶链反应（PCR）分析福尔马林固定及冷冻的儿童心肌炎、扩张型心肌病合并心内膜弹力纤维增生症心肌尸检样本中的病毒基因组。

Analysis of formalin-fixed and frozen myocardial autopsy samples for viral genome in childhood myocarditis and dilated cardiomyopathy with endocardial fibroelastosis using polymerase chain reaction (PCR).

作者信息

Griffin L D, Kearney D, Ni J, Jaffe R, Fricker F J, Webber S, Demmler G, Gelb B D, Towbin J A

机构信息

Department of Pediatrics, Baylor College of Medicine, Houston, Texas, U.S.A.; Institute for Molecular Genetics, Baylor College of Medicine, Houston, Texas, U.S.A.

Department of Pathology, Baylor College of Medicine, Houston, Texas, U.S.A.

出版信息

Cardiovasc Pathol. 1995 Jan-Mar;4(1):3-11. doi: 10.1016/1054-8807(94)00025-m.

DOI:10.1016/1054-8807(94)00025-m

PMID:25850773

Abstract

Viral infection of the myocardium is implicated in the pathogenesis of myocarditis and dilated cardiomyopathy (DCM). Enteroviruses have been considered the most common viral etiologic agents, based on peripheral culture and serologic methods. Recently, polymerase chain reaction (PCR) has been shown to be useful in the detection of viral genomes from various infected organs and body fluids. In this study, myocardial samples from autopsy specimens (formalin fixed and fresh frozen) were examined for enteroviral and DNA viral (adenovirus, herpes simplex virus [HSV], and cytomegalovirus (CMV]) genome by PCR. The specimens studied were from 58 patients with myocarditis, 28 patients with DCM and endocardial fibroelastosis [EFE], and 22 controls. Viral genome was detectable in 34 of the 58 (59%) autopsy-proven myocarditis samples (18 adenovirus, 12 enterovirus, 2 CMV, 2 HSV) and 6 of the 28 samples from patients with DCM and EFE (6 adenovirus). We conclude that PCR is effective in the rapid amplification of virus from frozen and formalin-fixed myocardial samples and that adenovirus is an important etiologic agent in viral myocarditis as well as DCM with EFE.

摘要

心肌的病毒感染与心肌炎和扩张型心肌病（DCM）的发病机制有关。基于外周培养和血清学方法，肠道病毒被认为是最常见的病毒病原体。最近，聚合酶链反应（PCR）已被证明可用于检测来自各种感染器官和体液的病毒基因组。在本研究中，通过PCR检测了尸检标本（福尔马林固定和新鲜冷冻）的心肌样本中的肠道病毒和DNA病毒（腺病毒、单纯疱疹病毒[HSV]和巨细胞病毒[CMV]）基因组。所研究的标本来自58例心肌炎患者、28例DCM合并心内膜弹力纤维增生症[EFE]患者和22例对照。在58例经尸检证实的心肌炎样本中有34例（59%）可检测到病毒基因组（18例腺病毒、12例肠道病毒、2例CMV、2例HSV），在28例DCM合并EFE患者的样本中有6例（6例腺病毒）可检测到病毒基因组。我们得出结论，PCR可有效地从冷冻和福尔马林固定的心肌样本中快速扩增病毒，并且腺病毒是病毒性心肌炎以及DCM合并EFE的重要病原体。