Erdogan Haluk, Arslan Hande
Departments of Infectious Disease and Clinical Microbiology, Faculty of Medicine, Baskent University, Ankara, Turkey,
Environ Monit Assess. 2015 May;187(5):235. doi: 10.1007/s10661-015-4444-3. Epub 2015 Apr 8.
This study evaluated the prevalence of Legionella species in water samples collected from Turkish baths in hotels in Alanya, Turkey, from August 2003 to September 2013. Water samples were collected in 100-mL sterile containers and then concentrated by filtration. Heat treatment was used to eliminate other microorganisms from the samples, which were then spread on Legionella-selective-buffered charcoal yeast extract alpha (BCYE-α) agar and on BCYE-α agar supplemented with glycine, vancomycin, polymyxin, and cycloheximide. Cysteine-dependent colonies were identified by latex agglutination. In total, 135 samples from 52 hotels with Turkish baths were evaluated. Legionella species were identified in 11/52 (21.2%) hotels and 18/135 (13.3%) samples. The most frequently isolated species was Legionella pneumophila, with most isolates belonging to serogroups 6 (55.6%) and 1 (22.2%). The colony count was <100 colony-forming units (CFU) mL(-1) in nine samples, from 100 to 1000 CFU mL(-1) in six samples, and >1000 CFU mL(-1) in three samples. These findings suggest that the hot water systems of Turkish baths in hotels must be viewed as a possible source of travel-associated Legionnaires' disease, and preventative measures should be put in place.
本研究评估了2003年8月至2013年9月期间从土耳其阿拉尼亚酒店的土耳其浴室采集的水样中军团菌属的流行情况。水样采集于100毫升无菌容器中，然后通过过滤进行浓缩。采用热处理去除样本中的其他微生物，随后将样本接种于军团菌选择性缓冲活性炭酵母浸出液α（BCYE-α）琼脂以及添加了甘氨酸、万古霉素、多粘菌素和环己酰亚胺的BCYE-α琼脂上。通过乳胶凝集试验鉴定半胱氨酸依赖型菌落。总共评估了来自52家设有土耳其浴室的酒店的135个样本。在11/52（21.2%）的酒店和18/135（13.3%）的样本中鉴定出军团菌属。最常分离出的菌种是嗜肺军团菌，大多数分离株属于血清型6（55.6%）和1（22.2%）。九个样本的菌落计数<100菌落形成单位（CFU）/毫升，六个样本的菌落计数为100至1000 CFU/毫升，三个样本的菌落计数>1000 CFU/毫升。这些发现表明，酒店土耳其浴室的热水系统必须被视为与旅行相关的军团病的一个可能来源，应采取预防措施。
