Lin Meng, Cai Shibin, Wang Shan, Liu Shubing, Zhang Guorong, Bai Guihua
Agronomy Department, Kansas State University, Manhattan, KS, 66506, USA.
Theor Appl Genet. 2015 Jul;128(7):1385-95. doi: 10.1007/s00122-015-2513-1. Epub 2015 Apr 8.
Using a GBS-SNP map, a QTL for pre-harvest sprouting resistance on 4AL of Totoumai A was delimited to 2.9-cM interval, and SNP closely linked to several other QTL were identified. Pre-harvest sprouting (PHS) of wheat is a major constraint to wheat production in many wheat-growing areas worldwide, because it reduces both wheat grain yield and the end-use quality. To identify markers tightly linked to the quantitative trait loci (QTL) for PHS resistance and seed dormancy (SD), we evaluated 155 recombinant inbred lines (RIL) derived from a cross between a PHS-resistant parent 'Tutoumai A' and a PHS-susceptible parent 'Siyang 936' for single-nucleotide polymorphisms (SNP) using genotyping-by-sequencing (GBS), and for PHS resistance and SD using both field and greenhouse grown plants. Two SNP, GBS109947 and GBS212432, were mapped to a major QTL region for PHS resistance and SD on chromosome 4AL, and delimited the QTL to a 2.9-cM interval. Two and nine additional SNP were mapped to minor QTL regions for SD on chromosome 5B and 5A, respectively. Critical SNP in these QTL regions were converted into KBioscience Competitive Allele-Specific PCR (KASP) assays that can be easily used for marker-assisted selection to improve PHS resistance.
利用GBS-SNP图谱，将秃头麦A 4AL染色体上一个抗穗发芽的QTL定位到2.9厘摩的区间内，并鉴定出与其他几个QTL紧密连锁的SNP。小麦穗发芽是全球许多小麦种植区小麦生产的主要限制因素，因为它会降低小麦产量和最终使用品质。为了鉴定与抗穗发芽和种子休眠的数量性状位点（QTL）紧密连锁的标记，我们利用简化基因组测序（GBS）对155个由抗穗发芽亲本“秃头麦A”和感穗发芽亲本“泗阳936”杂交得到的重组自交系（RIL）进行单核苷酸多态性（SNP）分析，并利用田间种植和温室种植的植株对抗穗发芽和种子休眠进行评估。两个SNP，GBS109947和GBS212432，被定位到4AL染色体上一个抗穗发芽和种子休眠的主要QTL区域，并将该QTL定位到2.9厘摩的区间内。另外分别有两个和九个SNP被定位到5B和5A染色体上种子休眠的次要QTL区域。这些QTL区域中的关键SNP被转化为KBioscience竞争性等位基因特异性PCR（KASP）分析方法，可轻松用于标记辅助选择以提高抗穗发芽能力。
