Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
College of Life Science, University of Chinese Academy of Science, Beijing, China.
Planta. 2019 Jul;250(1):187-198. doi: 10.1007/s00425-019-03164-9. Epub 2019 Apr 10.
Totally, 23 and 26 loci for the first count germination ratio and the final germination ratio were detected by quantitative trait loci (QTL) mapping and association mapping, respectively, which could be used to facilitate wheat pre-harvest sprouting breeding. Weak dormancy can cause pre-harvest sprouting in seeds of common wheat which significantly reduces grain yield. In this study, both quantitative trait loci (QTL) mapping and genome-wide association study (GWAS) were used to identify loci controlling seed dormancy. The analyses were based on a recombinant inbred line population derived from Zhou 8425B/Chinese Spring cross and 166 common wheat accessions. Inclusive composite interval mapping detected 8 QTL, while 45 loci were identified in the 166 wheat accessions by GWAS. Among these, four loci (Qfcgr.cas-3AS/Qfcgr.cas-3AS, Qfcgr.cas-6AL.1/Qfcgr.cas-6AL.1, Qfcgr.cas-7BL.2/Qfcgr.cas-7BL.2, and Qgr.cas-3DL/Qgr.cas-3DL) were detected in both QTL mapping and GWAS. In addition, 41 loci co-located with QTL reported previously, whereas 8 loci (Qfcgr.cas-5AL, Qfcgr.cas-6DS, Qfcgr.cas-7AS, Qgr.cas-3DS.1, Qgr.cas-3DS.2, Qgr.cas-3DL/Qgr.cas-3DL, Qgr.cas-4B, and Qgr.cas-5A) were likely to be new. Linear regression showed the first count germination ratio or the final germination ratio reduced while multiple favorable alleles increased. It is suggested that QTL pyramiding was effective to reduce pre-harvest sprouting risk. This study could enrich the research on pre-harvest sprouting and provide valuable information of marker exploration for wheat breeding programs.
总共，通过数量性状位点（QTL）作图和关联作图，分别检测到第一计数发芽率和最终发芽率的 23 个和 26 个位点，这些位点可用于促进小麦收获前发芽的育种。弱休眠会导致普通小麦种子在收获前发芽，从而显著降低谷物产量。在这项研究中，我们同时使用数量性状位点（QTL）作图和全基因组关联研究（GWAS）来鉴定控制种子休眠的位点。分析基于 Zhou 8425B/Chinese Spring 杂交重组自交系群体和 166 个普通小麦品种。包含性复合区间作图检测到 8 个 QTL，而 GWAS 在 166 个小麦品种中鉴定到 45 个位点。其中，在 QTL 作图和 GWAS 中均检测到 4 个位点（Qfcgr.cas-3AS/Qfcgr.cas-3AS、Qfcgr.cas-6AL.1/Qfcgr.cas-6AL.1、Qfcgr.cas-7BL.2/Qfcgr.cas-7BL.2 和 Qgr.cas-3DL/Qgr.cas-3DL）。此外，41 个位点与先前报道的 QTL 共定位，而 8 个位点（Qfcgr.cas-5AL、Qfcgr.cas-6DS、Qfcgr.cas-7AS、Qgr.cas-3DS.1、Qgr.cas-3DS.2、Qgr.cas-3DL/Qgr.cas-3DL、Qgr.cas-4B 和 Qgr.cas-5A）可能是新的。线性回归表明，随着多个有利等位基因的增加，第一计数发芽率或最终发芽率降低。这表明 QTL 聚合是降低收获前发芽风险的有效方法。这项研究丰富了收获前发芽的研究，并为小麦育种计划提供了有价值的标记探索信息。
