Rebelo Ana Rita, Carman Susy, Shapiro Jan, van Dreumel Tony, Hazlett Murray, Nagy Éva
Department of Pathobiology (Rebelo, Nagy) and Animal Health Laboratory, Laboratory Services Division (Rebelo, Carman, Shapiro, van Dreumel, Hazlett), Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1.
Can J Vet Res. 2015 Apr;79(2):155-9.
The objective of this study was to identify and partially characterize 3 equid herpesviruses that were isolated postmortem from zebras in Ontario, Canada in 1989, 2002, and 2007. These 3 virus isolates were characterized by plaque morphology, restriction fragment length polymorphism (RFLP) of their genomic deoxyribonucleic acid (DNA), real-time polymerase chain reaction (PCR) assay, and sequence analyses of the full length of the glycoprotein G (gG) gene (ORF70) and a portion of the DNA polymerase gene (ORF30). The isolates were also compared to 3 reference strains of equid herpesvirus 1 (EHV-1). Using rabbit kidney cells, the plaques for the isolates from the zebras were found to be much larger in size than the EHV-1 reference strains. The RFLP patterns of the zebra viruses differed among each other and from those of the EHV-1 reference strains. Real-time PCR and sequence analysis of a portion of the DNA polymerase gene determined that the herpesvirus isolates from the zebras contained a G at nucleotide 2254 and a corresponding N at amino acid position 752, which suggested that they could be neuropathogenic EHV-1 strains. However, subsequent phylogenetic analysis of the gG gene suggested that they were EHV-9 and not EHV-1.
本研究的目的是鉴定并部分表征1989年、2002年和2007年于加拿大安大略省从斑马尸体中分离出的3种马疱疹病毒。这3种病毒分离株通过蚀斑形态、其基因组脱氧核糖核酸（DNA）的限制性片段长度多态性（RFLP）、实时聚合酶链反应（PCR）分析以及糖蛋白G（gG）基因（开放阅读框70）全长和部分DNA聚合酶基因（开放阅读框30）的序列分析进行表征。这些分离株还与3种马疱疹病毒1型（EHV-1）参考毒株进行了比较。使用兔肾细胞，发现来自斑马的分离株形成的蚀斑比EHV-1参考毒株的蚀斑大得多。斑马病毒的RFLP模式彼此不同，且与EHV-1参考毒株的模式也不同。对部分DNA聚合酶基因的实时PCR和序列分析确定，来自斑马的疱疹病毒分离株在核苷酸2254处含有一个G，在氨基酸位置752处含有一个相应的N，这表明它们可能是神经致病性EHV-1毒株。然而，随后对gG基因的系统发育分析表明它们是EHV-9而非EHV-1。
