Borchers K, Frölich K
Institute of Virology, Free University Berlin, Germany.
J Wildl Dis. 1997 Oct;33(4):812-7. doi: 10.7589/0090-3558-33.4.812.
Twenty-one blood samples of free-ranging mountain zebras (Equus zebra) from Namibia were tested for equine herpesvirus (EHV-1, -2, -3, -4) specific antibodies by immunofluorescence assay (IFA) and neutralization test (NT). Additionally, type-specific nested polymerase chain reactions (nested PCR) were employed for detection of EHV-1, -2 and -4 DNA. Equine herpesvirus-1 antibodies were detected by IFA in all zebras, while only seven serum samples contained EHV-4 IFA antibodies. Sera with high IFA antibodies also were found to neutralize EHV-1 and -4. Furthermore, 20 zebras were EHV-2 seropositive by IFA, and one zebra had EHV-2 neutralizing antibodies. Equine herpesvirus-3 specific antibodies were not detected. We did not amplify EHV-1, -2 or -4 specific DNA sequences in peripheral blood leukocytes of the same zebras using type-specific nested PCR. EHV infections appear to be widespread in free-ranging zebras, as they are in domestic horses.
通过免疫荧光试验（IFA）和中和试验（NT）对来自纳米比亚的21份野生山地斑马（Equus zebra）血液样本进行了马疱疹病毒（EHV-1、-2、-3、-4）特异性抗体检测。此外，采用型特异性巢式聚合酶链反应（巢式PCR）检测EHV-1、-2和-4 DNA。通过IFA在所有斑马中均检测到马疱疹病毒1型抗体，而只有7份血清样本含有EHV-4 IFA抗体。IFA抗体水平高的血清也能中和EHV-1和-4。此外，20匹斑马通过IFA检测为EHV-2血清阳性，1匹斑马具有EHV-2中和抗体。未检测到马疱疹病毒3型特异性抗体。使用型特异性巢式PCR，我们未在同一批斑马的外周血白细胞中扩增出EHV-1、-2或-4特异性DNA序列。EHV感染在野生斑马中似乎很普遍，就像在家马中一样。
