Ciesielczuk Holly, Betts Jonathon, Phee Lynnette, Doumith Michel, Hope Russell, Woodford Neil, Wareham David W
a Antimicrobial Research Group; Center for Immunology and Infectious Disease; Blizard Institute, Barts and the London School of Medicine and Dentistry; Queen Mary; University of London ; London , UK.
Virulence. 2015;6(2):145-51. doi: 10.4161/21505594.2014.988095.
Extra-intestinal pathogenic Escherichia coli (ExPEC) are a significant cause of urinary tract infections and bacteraemia worldwide. Currently no single virulence factor or ExPEC lineage has been identified as the sole contributor to severe extra-intestinal infection and/or urosepsis. Galleria mellonella has recently been established as a simple model for studying the comparative virulence of ExPEC. In this study we investigated the virulence of 40 well-characterized ExPEC strains, in G. mellonella, by measuring mortality (larvae survival), immune recognition/response (melanin production) and cell damage (lactate dehydrogenase production). Although mortality was similar between urinary and bloodstream isolates, it was heightened for community-associated infections, complicated UTIs and urinary-source bacteraemia. Isolates of ST131 and those possessing afa/dra, ompT and serogroup O6 were also associated with heightened virulence.
肠外致病性大肠杆菌（ExPEC）是全球范围内尿路感染和菌血症的重要病因。目前，尚未确定单一的毒力因子或ExPEC菌系是导致严重肠外感染和/或泌尿道脓毒症的唯一因素。近来，大蜡螟已被确立为研究ExPEC比较毒力的简单模型。在本研究中，我们通过测量死亡率（幼虫存活情况）、免疫识别/反应（黑色素生成）和细胞损伤（乳酸脱氢酶生成），研究了40株特征明确的ExPEC菌株在大蜡螟中的毒力。尽管尿液和血流分离株的死亡率相似，但社区获得性感染、复杂性尿路感染和尿源性菌血症的死亡率更高。ST131分离株以及携带afa/dra、ompT和血清群O6的分离株也与毒力增强有关。
