在大蜡螟模型中肠道外致病性大肠杆菌尿液和血流分离株的比较毒力
Comparative virulence of urinary and bloodstream isolates of extra-intestinal pathogenic Escherichia coli in a Galleria mellonella model.
作者信息
Ciesielczuk Holly, Betts Jonathon, Phee Lynnette, Doumith Michel, Hope Russell, Woodford Neil, Wareham David W
机构信息
a Antimicrobial Research Group; Center for Immunology and Infectious Disease; Blizard Institute, Barts and the London School of Medicine and Dentistry; Queen Mary; University of London ; London , UK.
出版信息
Virulence. 2015;6(2):145-51. doi: 10.4161/21505594.2014.988095.
Abstract
Extra-intestinal pathogenic Escherichia coli (ExPEC) are a significant cause of urinary tract infections and bacteraemia worldwide. Currently no single virulence factor or ExPEC lineage has been identified as the sole contributor to severe extra-intestinal infection and/or urosepsis. Galleria mellonella has recently been established as a simple model for studying the comparative virulence of ExPEC. In this study we investigated the virulence of 40 well-characterized ExPEC strains, in G. mellonella, by measuring mortality (larvae survival), immune recognition/response (melanin production) and cell damage (lactate dehydrogenase production). Although mortality was similar between urinary and bloodstream isolates, it was heightened for community-associated infections, complicated UTIs and urinary-source bacteraemia. Isolates of ST131 and those possessing afa/dra, ompT and serogroup O6 were also associated with heightened virulence.
摘要
肠外致病性大肠杆菌（ExPEC）是全球范围内尿路感染和菌血症的重要病因。目前，尚未确定单一的毒力因子或ExPEC菌系是导致严重肠外感染和/或泌尿道脓毒症的唯一因素。近来，大蜡螟已被确立为研究ExPEC比较毒力的简单模型。在本研究中，我们通过测量死亡率（幼虫存活情况）、免疫识别/反应（黑色素生成）和细胞损伤（乳酸脱氢酶生成），研究了40株特征明确的ExPEC菌株在大蜡螟中的毒力。尽管尿液和血流分离株的死亡率相似，但社区获得性感染、复杂性尿路感染和尿源性菌血症的死亡率更高。ST131分离株以及携带afa/dra、ompT和血清群O6的分离株也与毒力增强有关。
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