Manosroi Aranya, Pattamapun Kassara, Khositsuntiwong Narinthorn, Kietthanakorn Bang-on, Issarangporn Witchapong, Chankhampan Charinya, Manosroi Worapaka, Manosroi Jiradej
Faculty of Pharmacy, Chiang Mai University , Chiang Mai , Thailand .
Pharm Biol. 2015;53(11):1653-60. doi: 10.3109/13880209.2014.1001402. Epub 2015 Apr 8.
Plant mucilages can be found in various parts of several Thai plants, which can be used as thickening, moisturizing, and lubricating agents in artificial saliva formulations.
The objective of this study was to evaluate the physicochemical properties, biological activity, and cytotoxicity of Thai plant mucilages.
The mucilages from Thai plants were extracted by various processes (temperature and pH variation, microwave oven, steam, and Tris-HCl buffer extraction). The viscosity and the rheology were evaluated using viscometer. Antioxidative activities including DPPH radical scavenging and metal chelating activities were investigated. The mucilages were determined for cytotoxicity on normal human gingival fibroblasts and anti-adherent activity of Streptococcus mutans.
Mucilages from Ocimum citriodorum Vis. (Lamiaceae), Artocarpus heterophyllus Lam. (Moraceae), Abelmoschus esculentus (Linn.) Moench. (Malvaceae), and Basella alba Linn. (Basellaceae) exhibited pseudoplastic non-Newtonian rheology. The highest DPPH radical-scavenging and metal-chelating activities were observed in the mucilages from B. alba (microwave, 3 min) and A. esculentus (microwave, 1 min) with the SC50 and MC50 values (50% of scavenging activity and 50% of metal chelating activity, respectively) of 0.71 ± 0.32 and 1.11 ± 0.52 mg/ml, respectively. Most mucilages exhibited no cytotoxicity to normal human gingival fibroblasts. The mucilage from A. esculentus (microwave, 5 min) gave the shortest wetting time of 2.75 ± 0.51 min. The highest S. mutans adhesion inhibition was observed in A. esculentus (pH 11) of 5.39 ± 9.70%.
This study has indicated the suitable physicochemical and biological properties and the potential application of mucilages from Thai plants for artificial saliva preparation.
植物黏液存在于多种泰国植物的不同部位，可作为人工唾液制剂中的增稠剂、保湿剂和润滑剂。
本研究旨在评估泰国植物黏液的理化性质、生物活性和细胞毒性。
通过各种工艺（温度和pH变化、微波炉、蒸汽和Tris-HCl缓冲液提取）从泰国植物中提取黏液。使用粘度计评估粘度和流变学。研究了包括DPPH自由基清除和金属螯合活性在内的抗氧化活性。测定了黏液对正常人牙龈成纤维细胞的细胞毒性和变形链球菌的抗黏附活性。
柠檬罗勒（唇形科）、木菠萝（桑科）、黄秋葵（锦葵科）和落葵（落葵科）的黏液表现出假塑性非牛顿流变学。在落葵（微波，3分钟）和黄秋葵（微波，1分钟）的黏液中观察到最高的DPPH自由基清除和金属螯合活性，其SC50和MC50值（分别为清除活性的50%和金属螯合活性的50%）分别为0.71±0.32和1.11±0.52mg/ml。大多数黏液对正常人牙龈成纤维细胞无细胞毒性。黄秋葵（微波，5分钟）的黏液湿润时间最短，为2.75±0.51分钟。在pH值为11的黄秋葵中观察到对变形链球菌黏附抑制作用最强，为5.39±9.70%。
本研究表明泰国植物黏液具有合适的理化和生物学性质，在人工唾液制备中具有潜在应用价值。
