Du Yong, Chen Xin, Lin Xiu-Qing, Wu Wei, Huang Zhi-Ming
Department of Pediatrics, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China E-mail :
Asian Pac J Cancer Prev. 2015;16(7):2665-9. doi: 10.7314/apjcp.2015.16.7.2665.
CD4+CD25+regulatory T cells (Tregs) play a key role in regulation of immnue response and maintenance of self-tolerance. Studies have found Tregs could suppress tumor-specific T cell-mediated immune response and promote cancer progression. Depletion of Tregs can enhance antitumor immunity. Dendritic cells (DCs) are professional antigen-presenting cells and capable of activating antigen-specific immune responses, which make them ideal candidate for cancer immunotherapy. Now various DC vaccines are considered as effective treatment for cancers. The aim of this study was to evaluate variation of Tregs in BALB/C mice with hepatocellular carcinoma and investigate the interaction between tumor-derived Tregs, effector T cells (Teff) and splenic DCs. We found the percentages of Tregs/CD4+ in the peripheral blood of tumor-bearing mice were higher than in normal mice. Tumor-derived Tregs diminished the up-regulation of costimulatory molecule expression on splenic DCs, even in the presence of Teff cells and simultaneously inhibited IL-12 and TNF-α secretion by DCs.
CD4+CD25+调节性T细胞（Tregs）在免疫反应调节和自身耐受性维持中起关键作用。研究发现，Tregs可抑制肿瘤特异性T细胞介导的免疫反应并促进癌症进展。清除Tregs可增强抗肿瘤免疫力。树突状细胞（DCs）是专业的抗原呈递细胞，能够激活抗原特异性免疫反应，这使其成为癌症免疫治疗的理想候选者。目前，各种DC疫苗被认为是治疗癌症的有效方法。本研究的目的是评估BALB/C肝癌小鼠中Tregs的变化，并研究肿瘤来源的Tregs、效应T细胞（Teff）和脾DCs之间的相互作用。我们发现荷瘤小鼠外周血中Tregs/CD4+的百分比高于正常小鼠。肿瘤来源的Tregs即使在存在Teff细胞的情况下也会减少脾DCs上共刺激分子表达的上调，并同时抑制DCs分泌IL-12和TNF-α。
