Melek Farouk R, Aly Fawzia A, Kassem Iman A A, Abo-Zeid Mona A M, Farghaly Ayman A, Hassan Zeinab M
Z Naturforsch C J Biosci. 2015;70(1-2):31-7. doi: 10.1515/znc-2014-4132.
Three triterpenoidal saponins were isolated from the saponin fraction derived from a Gleditsia caspica Desf. methanolic fruit extract. The isolated saponins were identified as gleditsiosides B, C, and Q based on spectral data. The saponin-containing fraction was evaluated in vivo for genotoxic and antigenotoxic activities. The fraction caused no DNA damage in Swiss albino male mice treated with a dose of 45 mg/kg body weight for 24 h, although it significantly inhibited the number of chromosomal aberrations induced by cyclophosphamide (CP) in bone marrow and germ cells when applied before or after CP administration. The inhibitory indices in chromosomal aberrations were 59% and 41% for bone marrow and 48% and 43% for germ cells, respectively. In addition, the saponin fraction was found to reduce the viability of the human tumor cell line MCF-7 in a dose-dependent manner with an extrapolated IC50 value in the range of 220 μg/mL.
从来源于皂荚（Gleditsia caspica Desf.）甲醇提取物的皂苷组分中分离出三种三萜皂苷。基于光谱数据，所分离出的皂苷被鉴定为皂荚皂苷B、C和Q。对含皂苷组分进行了体内遗传毒性和抗遗传毒性活性评估。该组分在以45 mg/kg体重剂量处理瑞士白化雄性小鼠24小时后未引起DNA损伤，尽管在环磷酰胺（CP）给药之前或之后应用时，它能显著抑制骨髓和生殖细胞中由环磷酰胺诱导的染色体畸变数量。骨髓中染色体畸变的抑制率分别为59%和41%，生殖细胞中为48%和43%。此外，发现皂苷组分以剂量依赖方式降低人肿瘤细胞系MCF-7的活力，外推IC50值在220 μg/mL范围内。
