Patel N U, Jameel S, Isom H, Siddiqui A
Department of Microbiology and Immunology, University of Colorado Medical School, Denver 80262.
J Virol. 1989 Dec;63(12):5293-301. doi: 10.1128/JVI.63.12.5293-5301.1989.
We have previously established that the tissue-specific activity of the hepatitis B virus (HBV) enhancer is mediated by trans-acting cellular factors. Here we have studied in vitro the interactions between the HBV enhancer DNA and cellular factors present in nuclear extracts from both liver and nonliver cell types. The results presented in this study imply the involvement of several distinct, ubiquitous, and liver-specific cellular factors with the HBV enhancer. Sequence analysis of the binding sites for these proteins on HBV DNA showed homologies to sequence motifs known to bind other previously characterized and purified transcription factors including CAAT/enhancer-binding protein. Thus, all of these binding sites may function in concert to activate liver-specific transcription of HBV genes from their respective promoters.
我们先前已经确定，乙型肝炎病毒（HBV）增强子的组织特异性活性是由反式作用细胞因子介导的。在此，我们在体外研究了HBV增强子DNA与肝脏和非肝脏细胞类型核提取物中存在的细胞因子之间的相互作用。本研究呈现的结果表明，几种不同的、普遍存在的和肝脏特异性的细胞因子参与了HBV增强子的作用。对这些蛋白质在HBV DNA上的结合位点进行序列分析，结果显示其与已知结合其他先前已鉴定和纯化的转录因子（包括CAAT/增强子结合蛋白）的序列基序具有同源性。因此，所有这些结合位点可能协同发挥作用，从各自的启动子激活HBV基因的肝脏特异性转录。
