Wang Y, Chen P, Wu X, Sun A L, Wang H, Zhu Y A, Li Z P
Shanghai Institute of Biochemistry, Academia Sinica, People's Republic of China.
J Virol. 1990 Aug;64(8):3977-81. doi: 10.1128/JVI.64.8.3977-3981.1990.
A new enhancer element of hepatitis B virus HBV, ENII, located in the X gene coding region and upstream of the C promoter, has been identified. As determined by deletion analysis, the sequence around nucleotides 1627 to 1732 was suggested to be essential for ENII activity. ENII was cell type specific. It showed high activity in HepG2 cells but not detectable activity in CV-1 cells. A protein-binding site was identified by footprinting in nucleotides 1648 to 1671. The minimum sequence and function of ENII are under investigation.
已鉴定出一种新的乙型肝炎病毒（HBV）增强子元件ENII，它位于X基因编码区且在C启动子上游。通过缺失分析确定，核苷酸1627至1732周围的序列被认为对ENII活性至关重要。ENII具有细胞类型特异性。它在HepG2细胞中显示出高活性，但在CV-1细胞中未检测到活性。通过足迹法在核苷酸1648至1671中鉴定出一个蛋白质结合位点。ENII的最小序列和功能正在研究中。
