Su Lian-lin, Cheng Xue, Ji De, Wang Li-jun, Ding Xi-yan, Lu Tu-lin, Mao Chun-qin, Zhang Ji
Yao Xue Xue Bao. 2016 Oct;51(10):1600-8.
UHPLC-QTOF/MS technique was used to study the differences of lignans and their metabolites derived from Schisandra chinensis and vinegar Schisandra chinensis in rat plasma, bile, urine and faeces by the data processing techniques such as the dynamic background subtract(DBS), mass defect filtering(MDF) and enhance peak list (EPL) in analysis. In order to enhance accuracy for Schisandra chinensis hepatoprotective effect, we established rat acute alcoholic liver injury model in this experiment, and studied the prototype components and metabolisms of Schisandra lignans in vivo under pathological condition. The main ingredients of alcohol extract are lignans, including deoxyschizandrin, schisandrin B, schizandrin C, schizandrol, schizandrol B,schisantherin, schisantherin B, schisanhenol, gomisin G, gomisin J. The metabolic transformation of lignans in rats was mainly induced by methylation, hydroxyl, oxidation, and so on. Finally, we identified 6 kinds of prototype components and their 20 potential metabolites in Schisandra chinensis group and vinegar Schisandra chinensis group.
采用超高效液相色谱-四极杆飞行时间质谱（UHPLC-QTOF/MS）技术，通过动态背景扣除（DBS）、质量亏损过滤（MDF）和增强峰列表（EPL）等数据处理技术，研究五味子及其醋制五味子在大鼠血浆、胆汁、尿液和粪便中木脂素及其代谢产物的差异。为提高五味子保肝作用研究的准确性，本实验建立大鼠急性酒精性肝损伤模型，研究病理状态下五味子木脂素的原型成分及其体内代谢情况。酒精提取物的主要成分是木脂素，包括脱氧五味子素、五味子醇乙、五味子丙素、五味子醇、五味子醇甲、华中五味子酯甲、华中五味子酯乙、华中五味子醇、戈米辛G、戈米辛J。大鼠体内木脂素的代谢转化主要由甲基化、羟基化、氧化等诱导。最终，我们在五味子组和醋制五味子组中鉴定出6种原型成分及其20种潜在代谢产物。
