Paranjpe Sarita S, Veenstra Gert Jan C
Radboud University, Department of Molecular Developmental Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands.
Radboud University, Department of Molecular Developmental Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Nijmegen, The Netherlands.
Biochim Biophys Acta. 2015 Jun;1849(6):626-36. doi: 10.1016/j.bbagrm.2015.03.006. Epub 2015 Apr 7.
The earliest steps of embryonic development involve important changes in chromatin and transcription factor networks, which are orchestrated to establish pluripotent cells that will form the embryo. DNA methylation, histone modifications, the pluripotency regulatory network of transcription factors, maternal factors and newly translated proteins all contribute to these transitions in dynamic ways. Moreover, these dynamics are linked to the onset of zygotic transcription. We will review recent progress in our understanding of chromatin state and regulation of gene expression in the context of embryonic development in vertebrates, in particular mouse, Xenopus and zebrafish. We include work on mouse embryonic stem cells and highlight work that illustrates how early embryonic dynamics establish gene regulatory networks and the state of pluripotency.
胚胎发育的最初阶段涉及染色质和转录因子网络的重要变化，这些变化协同作用以建立将形成胚胎的多能细胞。DNA甲基化、组蛋白修饰、转录因子的多能性调控网络、母体因子和新翻译的蛋白质都以动态方式促成这些转变。此外，这些动态变化与合子转录的起始相关。我们将综述在脊椎动物，特别是小鼠、非洲爪蟾和斑马鱼的胚胎发育背景下，我们对染色质状态和基因表达调控的理解方面的最新进展。我们纳入了关于小鼠胚胎干细胞的研究，并着重介绍了一些研究成果，这些成果阐明了早期胚胎动态变化如何建立基因调控网络和多能性状态。
