Li Hai, Chen Bingpu, Pang Guangfu, Chen Jianhai, Xie Jisheng, Huang Hailing
a Department of Histology and Embryology .
b Department of Anatomy , and.
Pharm Biol. 2016;54(1):111-7. doi: 10.3109/13880209.2015.1017885. Epub 2015 Apr 10.
Osteoporosis is one of the most common bone diseases, and radix of Pueraria lobata (Willd.) Ohwi possesses an obvious therapeutical effect on postmenopausal osteoporosis.
This study investigates the anti-osteoporotic activity of the puerarin 6"-O-xyloside (PXY) on ovariectomized mice and its related mechanism.
Osteoporotic mice model was established by ovariectomy (OVX). A total of 50 mice were divided into five groups (n = 10): sham, OVX group, PXY treatment groups (20, 40, and 60 mg/kg/d, i.p.). After 12 weeks' treatment, body weights were recorded. Then, mice were sacrificed, and serum samples were collected to determine the blood calcium, blood phosphorus, alkaline phosphatase (ALP), and osteoprotegerin (OPG) concentrations and uterine index was assayed. The thigh-bones of mice were collected to evaluate histopathological changes. In the in vitro experiment, the effect of PXY on osteoblasts' proliferation was evaluated and western blotting was performed to determine expressions of OPG and the receptor activators of NF-κB ligand (RANKL), as well as the ratio of OPG/RANKL.
PXY (40 and 60 mg/kg/d, i.p.) obviously decreased body weights and increased uterine index of OVX (p < 0.05), and improved osteoporotic syndromes of OVX mice; PXY also significantly increased the concentrations of blood calcium, blood phosphorus, ALP, and OPG of OVX mice (p < 0.05); moreover, PXY obviously up-regulated the ratio of OPG/RANKL (p < 0.05).
Our results demonstrated that the puerarin 6"-O-xyloside possesses significant anti-osteoporotic activity on ovariectomy mice.
骨质疏松症是最常见的骨疾病之一，葛根对绝经后骨质疏松症具有明显的治疗作用。
本研究探讨葛根素6″-O-木糖苷（PXY）对去卵巢小鼠的抗骨质疏松活性及其相关机制。
通过卵巢切除术（OVX）建立骨质疏松小鼠模型。将50只小鼠分为五组（n = 10）：假手术组、OVX组、PXY治疗组（20、40和60 mg/kg/d，腹腔注射）。治疗12周后，记录体重。然后，处死小鼠，收集血清样本以测定血钙、血磷、碱性磷酸酶（ALP）和骨保护素（OPG）浓度，并测定子宫指数。收集小鼠大腿骨以评估组织病理学变化。在体外实验中，评估PXY对成骨细胞增殖的影响，并进行蛋白质免疫印迹法以测定OPG和核因子κB受体活化因子配体（RANKL）的表达以及OPG/RANKL比值。
PXY（40和60 mg/kg/d，腹腔注射）明显降低OVX小鼠体重并增加子宫指数（p < 0.05），改善OVX小鼠的骨质疏松症状；PXY还显著增加OVX小鼠的血钙、血磷、ALP和OPG浓度（p < 0.05）；此外，PXY明显上调OPG/RANKL比值（p < 0.05）。
我们的结果表明，葛根素6″-O-木糖苷对去卵巢小鼠具有显著的抗骨质疏松活性。
