Zhou Qi-Lin, Qin Ru-Zi, Yang Yan-Xin, Huang Kun-Bing, Yang Xian-Wen
Department of Internal Medicine, Guangdong Province Corps Hospital, Chinese People's Armed Police Forces, Guangzhou, Guangdong 510507, P.R. China.
Mol Med Rep. 2016 Aug;14(2):1865-9. doi: 10.3892/mmr.2016.5432. Epub 2016 Jun 23.
This study was designed to investigate the anti‑osteoporotic activity of polydatin and its possible underlying mechanism. Osteoporosis was induced in mice by ovariectomy (OVX) and the mice were divided into 5 groups: An OVX only group, polydatin groups (10, 20 and 40 mg/kg) and a sham group (n=10/group). After 12 weeks of treatment, body weight, uterine index and the dry weight of thigh‑bones were recorded. In addition, the serum calcium, serum phosphorus, alkaline phosphatase (ALP) and osteoprotegerin (OPG) levels were also determined. Western blot analysis was then conducted to investigate the possible mechanism underlying the effect of polydatin via determining the expression of OPG, receptor activators of nuclear factor‑κB ligand (RANKL) and β‑catenin in the ST2 cell line. The results indicated that intraperitoneal injection of polydatin (10, 20 and 40 mg/kg/day) decreased body weight, and increased uterine index and dry weights of thigh‑bones of ovariectomized mice (P<0.05), and polydatin also significantly increased the serum calcium, phosphorus, ALP and OPG of ovariectomized mice (P<0.05). Results of western blot analysis showed that polydatin upregulated the ratio of OPG/RANKL (P<0.05) and β‑catenin protein in ST2 cells. In conclusion, the results demonstrated that polydatin exhibits anti‑osteoporotic activity via regulating osteoprotegerin, RANKL and β‑catenin.
本研究旨在探讨白藜芦醇苷的抗骨质疏松活性及其可能的潜在机制。通过卵巢切除术（OVX）诱导小鼠患骨质疏松症，并将小鼠分为5组：仅OVX组、白藜芦醇苷组（10、20和40mg/kg）和假手术组（每组n = 10）。治疗12周后，记录体重、子宫指数和大腿骨的干重。此外，还测定了血清钙、血清磷、碱性磷酸酶（ALP）和骨保护素（OPG）水平。然后进行蛋白质免疫印迹分析，通过测定ST2细胞系中OPG、核因子κB受体活化因子配体（RANKL）和β-连环蛋白的表达，探讨白藜芦醇苷作用的可能机制。结果表明，腹腔注射白藜芦醇苷（10、20和40mg/kg/天）可降低去卵巢小鼠的体重，并增加其子宫指数和大腿骨的干重（P<0.05），白藜芦醇苷还显著提高了去卵巢小鼠的血清钙、磷、ALP和OPG水平（P<0.05）。蛋白质免疫印迹分析结果显示，白藜芦醇苷上调了ST2细胞中OPG/RANKL的比值（P<0.05）和β-连环蛋白的表达。总之，结果表明白藜芦醇苷通过调节骨保护素、RANKL和β-连环蛋白发挥抗骨质疏松活性。
