Mert Sevda, Özbek Emin, Ötünçtemur Alper, Çulha Mustafa
Yeditepe University, Department of Genetics and Bioengineering, Faculty of Engineering, Kayisdagi Street, Atasehir, Istanbul 34755, Turkey.
Okmeydani Education and Research Hospital, Department of Urology, Istanbul 34360, Turkey.
J Biomed Opt. 2015 Apr;20(4):047002. doi: 10.1117/1.JBO.20.4.047002.
The detection of kidney cancers at an early stage is critical for diagnosis and therapy. Surface-enhanced Raman scattering (SERS) is investigated for early detection of cancer cases from biopsy samples. The colloidal silver nanoparticles as the SERS-active nanostructures are directly mixed with homogenized tissue samples. The SERS spectra from the normal and abnormal tissue samples collected from 40 cancer patients, 28 of them at T1 stage and 12 of them at T2–T3 stages, are analyzed using principal component analysis combined linear discriminant analysis with leave-one-out cross-validation method. It is found that the diagnosis sensitivity, specificity, and total accuracy of the approach can be as high as 100%. The results suggest that SERS can be used as a potential technique for the identification of the different tumor stages.
早期检测肾癌对于诊断和治疗至关重要。研究了表面增强拉曼散射（SERS）用于从活检样本中早期检测癌症病例。作为SERS活性纳米结构的胶体银纳米颗粒直接与匀浆后的组织样本混合。使用主成分分析结合线性判别分析和留一法交叉验证方法，对从40名癌症患者收集的正常和异常组织样本的SERS光谱进行了分析，其中28名处于T1期，12名处于T2 - T3期。结果发现该方法的诊断灵敏度、特异性和总准确率可高达100%。结果表明，SERS可作为一种潜在技术用于识别不同的肿瘤阶段。
