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黑种草籽油对大鼠乙醇毒性的影响。

Effect of Nigella sativa fixed oil on ethanol toxicity in rats.

作者信息

Pourbakhsh Hamed, Taghiabadi Elahe, Abnous Khalil, Hariri Alireza Timcheh, Hosseini Sayed Masoud, Hosseinzadeh Hossein

机构信息

Food Control Laboratory, Food and Drug Administration, Shiraz University of Medical Sciences, Shiraz, Iran.

Department of Phamacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.

出版信息

Iran J Basic Med Sci. 2014 Dec;17(12):1020-31.

PMID:25859307
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4387225/
Abstract

OBJECTIVES

This study was planned to appraise the protective effect of Nigella sativa fixed oil (NSO) against subchronic ethanol induced toxicity in rats.

MATERIALS AND METHODS

Studies were carried out on six groups of six animals each, including control (normal saline, gavage), ethanol (3 g/kg/day, gavage), NSO (0.125, 0.25 and 0.5 ml/Kg/day, IP) plus ethanol and NSO (0.5 ml/Kg/day, IP) groups. Treatments were continued for 4 weeks.

RESULTS

According to data, treatment with NSO attenuated ethanol-induced increased levels of malondialdehyde (MDA), tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6), as well as histopathological changes in liver and kidney tissues. Moreover, NSO improved the level of serum liver enzymes (including alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP) and glutathione (GSH) content in liver and kidney tissues in ethanol-treated rats. Western blot analysis and quantitative real time RT-PCR showed that NSO treatment inhibited apoptosis stimulated by ethanol through decreasing the Bax/Bcl-2 ratio (both protein and mRNA levels), cleaved caspase-3, cleaved caspase-8 and cleaved caspase-9 level in liver and kidney.

CONCLUSION

This study showed that NSO may have protective effects against hepatotoxicity and renal toxicity of ethanol by decreasing lipid peroxidation and inflammation and preventing apoptosis.

摘要

目的

本研究旨在评估黑种草籽油（NSO）对大鼠亚慢性乙醇诱导毒性的保护作用。

材料与方法

对六组动物进行研究，每组六只，包括对照组（生理盐水，灌胃）、乙醇组（3 g/kg/天，灌胃）、NSO组（0.125、0.25和0.5 ml/Kg/天，腹腔注射）加乙醇组以及NSO组（0.5 ml/Kg/天，腹腔注射）。治疗持续4周。

结果

根据数据，NSO治疗可减轻乙醇诱导的丙二醛（MDA）、肿瘤坏死因子α（TNF-α）和白细胞介素-6（IL-6）水平升高，以及肝脏和肾脏组织的组织病理学变化。此外，NSO改善了乙醇处理大鼠血清肝酶（包括丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）、碱性磷酸酶（ALP））水平以及肝脏和肾脏组织中的谷胱甘肽（GSH）含量。蛋白质印迹分析和实时定量RT-PCR显示，NSO治疗通过降低肝脏和肾脏中Bax/Bcl-2比值（蛋白质和mRNA水平）、裂解的半胱天冬酶-3、裂解的半胱天冬酶-8和裂解的半胱天冬酶-9水平，抑制了乙醇刺激的细胞凋亡。

结论

本研究表明，NSO可能通过减少脂质过氧化和炎症以及防止细胞凋亡，对乙醇的肝毒性和肾毒性具有保护作用。

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