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固定油的活性成分百里醌对大鼠乙醇毒性的保护作用。

Protective effect of thymoquinone, the active constituent of fixed oil, against ethanol toxicity in rats.

作者信息

Hosseini Sayed Masoud, Taghiabadi Elahe, Abnous Khalil, Hariri Alireza Timcheh, Pourbakhsh Hamed, Hosseinzadeh Hossein

机构信息

Department of Pharmacodynamy and Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.

出版信息

Iran J Basic Med Sci. 2017 Aug;20(8):927-939. doi: 10.22038/IJBMS.2017.9116.

DOI:10.22038/IJBMS.2017.9116
PMID:29085585
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5651479/
Abstract

OBJECTIVES

Long term consumption of ethanol may induce damage to many organs. Ethanol induces its noxious effects through reactive oxygen species production, and lipid peroxidation and apoptosis induction in different tissues and cell types. Previous experiments have indicated the antioxidant characteristics of thymoquinone, the active constituent of fixed oil, against biologically dangerous reactive oxygen species. This experiment was planned to evaluate the protective effect of thymoquinone against subchronic ethanol toxicity in rats.

MATERIALS AND METHODS

Experiments were performed on six groups. Each group consisted of six animals, including control group (saline, gavage), ethanol-receiving group (3 g/kg/day, gavage), thymoquinone (2.5, 5, 10 mg/Kg/day, intraperitoneally (IP)) plus ethanol and thymoquinone (10 mg/Kg/day, IP) groups. Treatments were carried out in four weeks.

RESULTS

Thymoquinone reduced the ethanol-induced increase in the lipid peroxidation and severity of histopathological alteration in liver and kidney tissues. In addition it improved the levels of proinflammatory cytokines in liver tissue. Furthermore, thymoquinone corrected the liver enzymes level including alanine transaminase, aspartate transaminase and alkaline phosphatase in serum and glutathione content in liver and kidney tissues. Other experiments such as Western blot analysis and quantitative real-time RT-PCR revealed that thymoquinone suppressed the expression of Bax/Bcl-2 ratio (both protein and mRNA level), and caspases activation pursuant to ethanol toxicity.

CONCLUSION

This study indicates that thymoquinone may have preventive effects against ethanol toxicity in the liver and kidney tissue through reduction in lipid peroxidation and inflammation, and also interrupting apoptosis.

摘要

目的

长期摄入乙醇可能会对多个器官造成损害。乙醇通过产生活性氧、脂质过氧化以及在不同组织和细胞类型中诱导凋亡来产生有害影响。先前的实验表明，黑种草子油的活性成分百里醌具有对抗生物危险性活性氧的抗氧化特性。本实验旨在评估百里醌对大鼠亚慢性乙醇毒性的保护作用。

材料与方法

实验分为六组。每组有六只动物，包括对照组（生理盐水，灌胃）、乙醇摄入组（3克/千克/天，灌胃）、百里醌（2.5、5、10毫克/千克/天，腹腔注射）加乙醇组以及百里醌（10毫克/千克/天，腹腔注射）组。处理持续四周。

结果

百里醌降低了乙醇诱导的肝和肾组织脂质过氧化增加以及组织病理学改变的严重程度。此外，它改善了肝组织中促炎细胞因子的水平。此外，百里醌纠正了血清中谷丙转氨酶、谷草转氨酶和碱性磷酸酶等肝酶水平以及肝和肾组织中的谷胱甘肽含量。其他实验如蛋白质印迹分析和定量实时逆转录聚合酶链反应显示，百里醌抑制了乙醇毒性导致的Bax/Bcl - 2比值（蛋白质和mRNA水平）的表达以及半胱天冬酶的激活。

结论

本研究表明，百里醌可能通过减少脂质过氧化和炎症以及中断凋亡，对肝和肾组织的乙醇毒性具有预防作用。

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