Ashwin Devasya, Ke Vijayaprasad, Taranath Mahanthesh, Ramagoni Naveen Kumar, Nara Asha, Sarpangala Mythri
Senior Lecturer, Department of Pediatric and Preventive Dentistry, Kannur Dental College , Anjarakandy, Kannur, Kerala, India .
Professor & Head, Department of Pediatric and Preventive Dentistry, Navodaya Dental College , Raichur, Karnataka, India .
J Clin Diagn Res. 2015 Feb;9(2):ZC06-9. doi: 10.7860/JCDR/2015/10942.5532. Epub 2015 Feb 1.
To evaluate the caries risk based on the salivary levels of streptococcus mutans in children of 6-12 years of age group before and after consuming probiotic ice-cream containing Bifidobacterium lactis Bb-12 and Lactobacillus acidophilus La-5.
A double blind, placebo controlled trial was carried out in 60 children aged between 6 to 12 years with zero decayed, missing, and filled teeth (DMFT). They were randomly divided into two equal groups. Saliva sample were collected before the consumption of ice-cream and Streptococcus mutans count was calculated and recorded as baseline data. For the next seven days both the groups were given ice creams marked as A and B. Saliva samples were collected after ice-cream consumption at the end of study period and also after a washout period of 30 days and again after six months. Samples were inoculated and colonies were counted.
On statistical evaluation by students paired t-test, probiotic ice-cream brought significant reduction in the Streptococcus mutans count after seven days of ice-cream ingestion (p<0.001) and also after 30 d of washout period (p<0.001). There was no significant reduction (p=0.076) by normal ice-cream consumption. After six months of the study period in both the groups the salivary levels of Streptococcus mutans was similar to the baseline.
Probiotic ice-cream containing Bifidobacterium lactis Bb-12 and Lactobacillus acidophilus La-5 can cause reduction in caries causative organism. The dosage of the probiotic organisms for the long term or synergetic effect on the oral health are still needed to be explored.
基于6至12岁儿童食用含乳酸双歧杆菌Bb-12和嗜酸乳杆菌La-5的益生菌冰淇淋前后唾液中变形链球菌水平，评估龋齿风险。
对60名年龄在6至12岁、乳牙龋失补牙面数（DMFT）为零的儿童进行双盲、安慰剂对照试验。他们被随机分为两组。在食用冰淇淋前收集唾液样本，计算并记录变形链球菌计数作为基线数据。在接下来的七天里，两组儿童都食用标为A和B的冰淇淋。在研究期结束时食用冰淇淋后、30天的洗脱期后以及六个月后再次收集唾液样本。接种样本并计数菌落。
通过学生配对t检验进行统计学评估，食用益生菌冰淇淋七天后（p<0.001）以及30天洗脱期后（p<0.001），变形链球菌计数显著降低。食用普通冰淇淋则无显著降低（p=0.076）。在研究期六个月后，两组唾液中变形链球菌水平与基线相似。
含乳酸双歧杆菌Bb-12和嗜酸乳杆菌La-5的益生菌冰淇淋可使致龋菌数量减少。益生菌长期使用的剂量或对口腔健康的协同作用仍有待探索。
