Zeng Zhikai, Li Qingyun, Tian Qiyu, Zhao Panfeng, Xu Xiao, Yu Shukun, Piao Xiangshu
State Key Laboratory of Animal Nutrition, Ministry of Agriculture Feed Industry Centre, China Agricultural University, No. 2. West Yuanmingyuan Road, Beijing, 100193, China.
Department of Biotechnology, Lund University, Lund, Sweden.
Biol Trace Elem Res. 2015 Nov;168(1):103-9. doi: 10.1007/s12011-015-0319-2. Epub 2015 Apr 11.
This study was conducted to evaluate the efficacy of a novel Buttiauxella phytase to pigs fed P-deficient, corn-soybean meal diets. One hundred and twenty crossbred piglets (9.53 ± 0.84 kg) were allocated to one of five treatments which consisted of four low P diets (0.61 % Ca and 0.46 % total P) supplemented with 0, 500, 1,000, or 20,000 FTU/kg phytase as well as a positive control diet (0.77 % Ca and 0.62 % total P). Each treatment had six replicated pens with four pigs per pen. Pigs were fed the experimental diets for 28 days. Phytase supplementation linearly improved (P < 0.05) average daily gain (ADG), feed conversion ratio (FCR), and apparent total tract digestibility (ATTD) of dry matter, gross energy, crude protein, Ca, and P in weaned pigs. Super high dosing with phytase (20,000 FTU/kg) further increased (P < 0.05) ADG compared with 500 FTU/kg phytase inclusion group, as well as ATTD of Ca and P. Metacarpal bone characteristics and several trace mineral concentration in bone, plasma, or organ tissues were linearly (P < 0.05) improved at increasing dose of phytase. Super high dosing with phytase (20,000 FTU/kg) supplementation improved (P < 0.05) Mn and Zn concentration in bone compared to normal dose of phytase supplementation (500 or 1,000 FTU/kg). In conclusion, supplementation of 500 FTU of Buttiauxella phytase/kg and above effectively hydrolyzed phytate in a low-P corn-soybean diet for pigs. In addition, a super high dosing with phytase (20,000 FTU/kg) improved macro- or micro mineral availability and growth performance.
本研究旨在评估一种新型布氏植酸酶对饲喂低磷玉米 - 豆粕日粮的猪的功效。120头杂交仔猪（9.53±0.84千克）被分配到五种处理之一，其中四种低磷日粮（0.61%钙和0.46%总磷）分别添加0、500、1000或20000 FTU/千克植酸酶，以及一种正对照日粮（0.77%钙和0.62%总磷）。每个处理有六个重复栏，每栏四头猪。猪饲喂实验日粮28天。添加植酸酶线性改善了（P<0.05）断奶仔猪的平均日增重（ADG）、饲料转化率（FCR）以及干物质、总能、粗蛋白、钙和磷的表观全肠道消化率（ATTD）。与添加500 FTU/千克植酸酶组相比，超高剂量（20000 FTU/千克）添加植酸酶进一步提高了（P<0.05）ADG以及钙和磷的ATTD。随着植酸酶剂量增加，掌骨特性以及骨、血浆或器官组织中几种微量元素浓度呈线性改善（P<0.05）。与正常剂量（500或1000 FTU/千克）添加植酸酶相比，超高剂量（20000 FTU/千克）添加植酸酶提高了（P<0.05）骨中锰和锌的浓度。总之，每千克日粮添加500 FTU及以上的布氏植酸酶可有效水解低磷玉米 - 豆粕日粮中的植酸盐。此外，超高剂量（20000 FTU/千克）添加植酸酶可提高常量或微量矿物质利用率以及生长性能。
