Moghaddam Arash, Child Christopher, Bruckner Thomas, Gerner Hans Jürgen, Daniel Volker, Biglari Bahram
Heidelberg Trauma Research Group, Trauma and Reconstructive Surgery, Center for Orthopedics, Trauma Surgery and Spinal Cord Injury, Heidelberg University Hospital, Schlierbacher Landstraße 200a, D-69118 Heidelberg, Germany.
Institute for Medical Biometry and Informatics, Heidelberg University Hospital, Im Neuenheimer Feld 305, D-69120 Heidelberg, Germany.
Int J Mol Sci. 2015 Apr 9;16(4):7900-16. doi: 10.3390/ijms16047900.
Pro- and anti-inflammatory cytokines might have a large impact on the secondary phase and on the neurological outcome of patients with acute spinal cord injury (SCI). We measured the serum levels of different cytokines (Interferon-γ, Tumor Necrosis Factor-α, Interleukin-1β, IL-6, IL-8, IL-10, and Vascular Endothelial Growth Factor) over a 12-week period in 40 acute traumatic SCI patients: at admission on average one hour after initial trauma; at four, nine, 12, and 24 h; Three, and seven days after admission; and two, four, eight, and twelve weeks after admission. This was done using a Luminex Performance Human High Sensitivity Cytokine Panel. SCI was classified using the American Spinal Injury Association (ASIA) Impairment Scale (AIS) at time of admission and after 12 weeks. TNFα, IL-1β, IL-6, IL-8, and IL-10 concentrations were significantly higher in patients without neurological remission and in patients with an initial AIS A (p < 0.05). This study shows significant differences in cytokine concentrations shown in traumatic SCI patients with different neurological impairments and within a 12-week period. IL-8 and IL-10 are potential peripheral markers for neurological remission and rehabilitation after traumatic SCI. Furthermore our cytokine expression pattern of the acute, subacute, and intermediate phase of SCI establishes a possible basis for future studies to develop standardized monitoring, prognostic, and tracking techniques.
促炎细胞因子和抗炎细胞因子可能对急性脊髓损伤（SCI）患者的继发阶段及神经功能预后产生重大影响。我们在40例急性创伤性SCI患者中，于12周内测量了不同细胞因子（干扰素-γ、肿瘤坏死因子-α、白细胞介素-1β、IL-6、IL-8、IL-10和血管内皮生长因子）的血清水平：在入院时，即初始创伤后平均1小时；在4、9、12和24小时；入院后3天和7天；以及入院后2、4、8和12周。这是使用Luminex性能人高灵敏度细胞因子检测板完成的。在入院时和12周后，使用美国脊髓损伤协会（ASIA）损伤量表（AIS）对SCI进行分类。在无神经功能恢复的患者和初始AIS为A的患者中，TNFα、IL-1β、IL-6、IL-8和IL-10的浓度显著更高（p<0.05）。本研究显示，不同神经功能损伤的创伤性SCI患者在12周内细胞因子浓度存在显著差异。IL-8和IL-1０是创伤性SCI后神经功能恢复和康复的潜在外周标志物。此外，我们对SCI急性期、亚急性期和中期的细胞因子表达模式为未来开发标准化监测、预后和跟踪技术的研究奠定了可能的基础。
