Salim-Hanna M, Campa A, Cilento G
Department of Biochemistry, Instituto de Quimica, Universidade de São Paulo, Brazil.
Lipids. 1989 Aug;24(8):750-2. doi: 10.1007/BF02535217.
Combined--but not individual--microsomal and supernatant fractions obtained from rat brains not only consume oxygen but also provoke emission from added chlorophyll. These results are consistent with literature data (Levis and Mead, J. Biol. Chem. 239, 77 [1964]) for trapping of radioactive 14CO2 following addition of alpha-hydroxy-[1-14C]stearic acid. The most plausible explanation for emission is the interaction of chlorophyll with an alpha-peroxylactone. An intermediary alpha-peroxylactone in alpha-oxidation is consistent with other available data (Salim-Hanna, Campa and Cilento, Photochem. Photobiol. 45, 849 [1987]; Campa, Salim-Hanna and Cilento, Photochem. Photobiol. 49, 349 [1989]) and, on chemical grounds, provides a feasible route to the final products.
从大鼠大脑中获得的微粒体和上清液组分混合后（而非单独的组分）不仅消耗氧气，还会促使添加的叶绿素发光。这些结果与文献数据（莱维斯和米德，《生物化学杂志》239卷，77页 [1964年]）一致，即在添加α-羟基-[1-¹⁴C]硬脂酸后捕获放射性¹⁴CO₂ 。发光最合理的解释是叶绿素与α-过氧内酯的相互作用。α-氧化过程中的中间α-过氧内酯与其他现有数据（萨利姆-汉纳、坎帕和奇伦托，《光化学与光生物学》45卷，849页 [1987年]；坎帕、萨利姆-汉纳和奇伦托，《光化学与光生物学》49卷，349页 [1989年]）一致，并且从化学角度来看，为最终产物提供了一条可行的途径。
