Lissi E A, Cáceres T, Videla L A
Departamento de Química, Facultad de Ciencias, Universidad de Santiago, Chile.
Free Radic Biol Med. 1988;4(2):93-7. doi: 10.1016/0891-5849(88)90069-x.
The visible luminescence emitted in the autoxidation of brain homogenates is only partially quenched when antioxidants are added at concentrations such that further oxidation is prevented. From the time course of the emission after antioxidant addition, it can be estimated that nearly 50% of the light arises from an intermediate that decays with a first order kinetics and with a lifetime of ca. 40 s at 32 degrees C. The remaining light arises from the decomposition of one or several intermediates, and show a kinetics that is independent of the incubation time. From the data obtained it is concluded that bimolecular free radical processes, such as the recombination of peroxy radicals, do not significantly contribute to the observed luminescence.
当以足以防止进一步氧化的浓度添加抗氧化剂时，脑匀浆自氧化过程中发出的可见发光仅被部分淬灭。从添加抗氧化剂后发光的时间进程可以估计，近50%的光来自一种中间体，该中间体以一级动力学衰减，在32℃下的寿命约为40秒。其余的光来自一种或几种中间体的分解，其动力学与孵育时间无关。根据获得的数据得出结论，双分子自由基过程，如过氧自由基的重组，对观察到的发光没有显著贡献。
