Kim Julian O, Gazala Sayf, Razzak Rene, Guo Linghong, Ghosh Sunita, Roa Wilson H, Bédard Eric L R
Department of Oncology, University of Alberta, Edmonton, Alberta, Canada Division of Radiation Oncology, Department of Radiology, University of Manitoba, Winnipeg, Manitoba, Canada Cross Cancer Institute, Edmonton, Alberta, Canada CancerCare, Winnipeg, Manitoba, Canada.
Division of Thoracic Surgery, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada.
Anticancer Res. 2015 Apr;35(4):1873-80.
To assess if miRNA expression profiling of bronchoalveolar lavage (BAL) fluid and sputum could be used to detect early-stage non-small cell lung cancer (NSCLC).
Hierarchical cluster analysis was performed on the expression levels of 5 miRNAs (miR-21, miR-143, miR-155, miR-210, and miR-372) which were quantified using RNA reverse transcription and quantitative real-time polymerase chain reaction in sputum and BAL samples from NSCLC cases and cancer-free controls.
Cluster analysis of the miRNA expression levels in BAL samples from 21 NSCLC cases and sputum samples from 10 cancer-free controls yielded a diagnostic sensitivity of 85.7% and specificity of 100%. Cluster analysis of sputum samples from the same patients yielded a diagnostic sensitivity of 67.8% and specificity of 90%.
miRNA expression profiling of sputum and BAL fluids represent a potential means to detect early-stage NSCLC.
评估支气管肺泡灌洗（BAL）液和痰液的微小RNA（miRNA）表达谱是否可用于检测早期非小细胞肺癌（NSCLC）。
对5种miRNA（miR-21、miR-143、miR-155、miR-210和miR-372）的表达水平进行层次聚类分析，这些miRNA在NSCLC病例和无癌对照的痰液及BAL样本中通过RNA逆转录和定量实时聚合酶链反应进行定量。
对21例NSCLC病例的BAL样本和10例无癌对照的痰液样本中的miRNA表达水平进行聚类分析，诊断敏感性为85.7%，特异性为100%。对同一患者的痰液样本进行聚类分析，诊断敏感性为67.8%，特异性为90%。
痰液和BAL液的miRNA表达谱是检测早期NSCLC的一种潜在手段。
