Institute of Psychology and Behavioral Neuroscience, University of Tsukuba, Tsukuba, Ibaraki 305-8577, Japan.
Graduate School of Literature and Human Sciences, Osaka City University, Sumiyoshi-ku, Osaka 558-8585, Japan.
Eur J Pharmacol. 2015 Jul 5;758:137-41. doi: 10.1016/j.ejphar.2015.03.080. Epub 2015 Apr 9.
The possible involvement of hippocampal N-methyl-d-aspartate (NMDA) receptors in spatial reference and working memory was investigated. Rats were first trained in a four-baited/four-unbaited version of the eight-arm radial maze task in which only predetermined four arms for each rat were baited with a food pellet. After rats reached the learning criterion, their performance was tested under the treatment of a NMDA antagonist, AP5 (d,l-2-amino-5-phosphonopentanoic acid, 20-40nmol), or vehicle into the dorsal hippocampus through the bilaterally implanted guide cannulae. AP5 produced dose-dependent increments on both reference and working memory errors, but did not have any effect on the running speed. Additionally, there were significant correlations between the number of trials to criterion in acquisition and the number of reference and working memory errors induced by AP5 treatment. The results suggest that hippocampal NMDA receptors are involved in both spatial reference and working memory.
研究了海马 N-甲基-D-天冬氨酸 (NMDA) 受体在空间参照和工作记忆中的可能作用。首先，大鼠在一个有四个诱饵/四个无诱饵的八臂放射状迷宫任务中接受训练，对于每只大鼠，只有预定的四个臂有食物丸作为诱饵。当大鼠达到学习标准后，通过双侧植入的导向套管，将 NMDA 拮抗剂 AP5（d,l-2-氨基-5-膦戊酸，20-40nmol）或载体注入其海马背侧，然后对它们的表现进行测试。AP5 对参考和工作记忆错误均产生了剂量依赖性的增加，但对奔跑速度没有任何影响。此外，在获得标准的试验次数与 AP5 处理引起的参考和工作记忆错误数量之间存在显著相关性。这些结果表明，海马 NMDA 受体参与了空间参照和工作记忆。
