Kawabe K, Ichitani Y, Iwasaki T
Institute of Psychology, University of Tsukuba, Tsukuba, Ibaraki 305, Japan.
Brain Res. 1998 Jan 19;781(1-2):300-6. doi: 10.1016/s0006-8993(97)01256-0.
The purpose of the present study was to investigate the possible involvement of hippocampal NMDA (N-methyl-d-aspartate) receptors in spatial memory in rats by means of intrahippocampal injection of an NMDA antagonist, AP5 (D,L-2-amino-5-phosphonopentanoic acid). In Expt. 1, spontaneous motor activity (locomotor activity and rearing) was measured in an open-field after bilateral AP5 injection into the hippocampus, and it was shown that AP5 did not affect general activities within the dosage used in the present study. In Expt. 2, radial-maze performance was observed after the intrahippocampal injection of AP5. Rats were required to consume all the pellets which were put on each of the eight arms of the maze without revisiting the arms. AP5 produced dose-dependent impairment on the choice performance in the radial-arm maze, but did not have any effect on their running time. These results suggest that hippocampal NMDA receptors are involved in spatial memory.
本研究的目的是通过向大鼠海马内注射N-甲基-D-天冬氨酸（NMDA）拮抗剂AP5（D，L-2-氨基-5-磷酸戊酸），来研究海马NMDA受体在大鼠空间记忆中可能发挥的作用。在实验1中，向海马双侧注射AP5后，在旷场中测量自发运动活动（运动活性和竖毛行为），结果表明，在本研究使用的剂量范围内，AP5不影响一般活动。在实验2中，海马内注射AP5后观察放射状迷宫表现。要求大鼠吃光放置在迷宫八个臂上的所有颗粒，且不能再次进入已去过的臂。AP5对放射状臂迷宫中的选择表现产生剂量依赖性损害，但对其奔跑时间没有任何影响。这些结果表明，海马NMDA受体参与空间记忆。
