Bozok University, Faculty of Arts and Science, Department of Biology, 66100 Yozgat, Turkey.
Gazi University, Faculty of Science, Department of Biology, 06500 Ankara, Turkey.
Environ Toxicol Pharmacol. 2015 May;39(3):1019-26. doi: 10.1016/j.etap.2015.03.012. Epub 2015 Mar 27.
The adverse effects of lead nitrate (LN) and the preventive role of sodium selenite were investigated in diabetic and non-diabetic rat blood by measuring trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant power (FRAP), malondialdehyde (MDA) levels and activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) also by evaluating DNA damage with comet assay. LN increased the levels of MDA, tail DNA%, mean tail length and tail moment, decreased the enzymes activities, FRAP and TEAC values. In sodium selenite+LN group, we observed the protective effect of sodium selenite on examining parameters. Diabetes caused alterations on these parameters, too. We found that sodium selenite did not protect against diabetes caused damages. As a result, LN caused toxic effects on blood cells and sodium selenite alleviated this toxicity but it did not show preventive effect against diabetes. Also, LN caused more harmfull effects in diabetic groups than non-diabetic groups.
研究了硝酸铅（LN）的不良反应和亚硒酸钠的预防作用，通过测量 Trolox 等效抗氧化能力（TEAC）、铁还原抗氧化能力（FRAP）、丙二醛（MDA）水平以及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和谷胱甘肽-S-转移酶（GST）的活性，还通过彗星试验评估了 DNA 损伤。LN 增加 MDA、尾部 DNA%、平均尾部长度和尾部矩，降低酶活性、FRAP 和 TEAC 值。在亚硒酸钠+LN 组中，我们观察到亚硒酸钠对检查参数的保护作用。糖尿病也对这些参数产生了改变。我们发现亚硒酸钠不能预防糖尿病引起的损伤。因此，LN 对血细胞造成了毒性作用，而亚硒酸钠减轻了这种毒性，但对糖尿病没有预防作用。此外，LN 在糖尿病组中造成的危害比非糖尿病组更大。
