School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China.
Talanta. 2015 Jun 1;138:40-45. doi: 10.1016/j.talanta.2015.02.003. Epub 2015 Feb 9.
Ritodrine has similar skeleton structure to ractopamine and it was selected as the dummy-template molecule to synthesize the molecular imprinted polymers (MIPs). The MIPs exhibited better selectivity to ractopamine than to the dummy-template molecule: the imprint factor for ractopamine was 8.9, while 7.6 for ritodrine. The MIPs were used as sorbents in solid-phase extraction for selective enrichment of ractopamine, and some key parameters were optimized. After that, a rapid surface-enhanced Raman spectroscopy method was developed for analysis of ractopamine and isoxuprine in pig tissue samples. Under the optimal conditions, good linearity was achieved in the range of 20.0-200.0 μg/L for ractopamine and isoxsuprine at 842 cm(-1) and 993 cm(-1), respectively. The limits of detection were 3.1-4.3 μg/L, which were lower than the maximum allowed by U. S. Food and Drug Administration. The recoveries of ractopamine and isoxsuprine were 72.4-79.7% and 71.0-78.2% for the spiked pork and pig liver, respectively, while the relative standard deviations ranging from 7.4% to 13.0%. The results suggest that the proposed method is sensitive and selective, and it has good potential on the quantitative analysis of trace amounts of β-agonists in complex samples.
利托君与莱克多巴胺具有相似的骨架结构，因此被选为合成分子印迹聚合物（MIPs）的虚拟模板分子。MIPs 对莱克多巴胺的选择性优于虚拟模板分子：莱克多巴胺的印迹因子为 8.9，而利托君为 7.6。MIPs 被用作固相萃取中的吸附剂，用于选择性富集莱克多巴胺，并对一些关键参数进行了优化。然后，建立了一种快速表面增强拉曼光谱法，用于分析猪组织样品中的莱克多巴胺和异噁唑啉。在最佳条件下，在 20.0-200.0 μg/L 范围内，莱克多巴胺和异噁唑啉在 842 cm(-1) 和 993 cm(-1) 处均具有良好的线性关系。莱克多巴胺和异噁唑啉的检出限分别为 3.1-4.3 μg/L，低于美国食品和药物管理局规定的最大值。莱克多巴胺和异噁唑啉在猪肉和猪肝中的加标回收率分别为 72.4-79.7%和 71.0-78.2%，相对标准偏差在 7.4%-13.0%之间。结果表明，该方法灵敏、选择性好，在复杂样品中痕量 β-激动剂的定量分析中具有良好的应用潜力。
