Hui Minyi, Ma Xi, Yuan Jiayu, Wang Zhouping, Ma Xiaoyuan
State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, 214122, China.
School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
Anal Bioanal Chem. 2025 Mar;417(6):1127-1138. doi: 10.1007/s00216-024-05714-x. Epub 2025 Jan 10.
Ofloxacin is a commonly used quinolone antibiotic that is also used as a feed supplement in livestock production and in plant disease prevention and treatment. However, the excessive use and abuse of ofloxacin will accumulate along the food chain and endanger human health. Therefore, the development of a simple, rapid, and sensitive detection method for the determination of ofloxacin is critical. Herein, a detection method combining molecularly imprinted magnetic solid-phase extraction (MISPE) and surface-enhanced Raman spectroscopy (SERS) was developed for the detection of ofloxacin. Graphene oxide supported by magnetic beads was synthesized by a one-pot method, producing what was subsequently referred to as magnetic graphene oxide (MGO), and a molecularly imprinted membrane was synthesized on its surface by exploiting the ability of dopamine to self-polymerize under alkaline conditions. MGO@MIPs were obtained as the adsorbent for magnetic solid-phase extraction, which was used for the extraction and enrichment of ofloxacin in complex sample matrix, and then quantitative analysis was conducted by SERS. The developed method exhibited an excellent linear relationship with respect to ofloxacin concentration (10 to 10 μg mL), with a detection limit of 9.7 × 10 μg mL in ultrapure water. Blank honey, milk, and pork samples spiked with ofloxacin at concentrations of 0.005, 0.1, 1, and 10 μg mL were extracted and determined using the developed method, with recoveries ranging from 93.1% to 105.6%. The results support the strong application prospects for the method, demonstrating simple and time-efficient operation and high accuracy.
氧氟沙星是一种常用的喹诺酮类抗生素，也用作畜牧生产中的饲料添加剂以及植物疾病防治。然而，氧氟沙星的过度使用和滥用会沿食物链累积并危害人类健康。因此，开发一种简单、快速且灵敏的氧氟沙星检测方法至关重要。在此，开发了一种结合分子印迹磁性固相萃取（MISPE）和表面增强拉曼光谱（SERS）的检测方法用于氧氟沙星的检测。通过一锅法合成了磁珠负载的氧化石墨烯，即随后所称的磁性氧化石墨烯（MGO），并利用多巴胺在碱性条件下的自聚合能力在其表面合成了分子印迹膜。获得了MGO@MIPs作为磁性固相萃取的吸附剂，用于复杂样品基质中氧氟沙星的萃取和富集，然后通过SERS进行定量分析。所开发的方法在氧氟沙星浓度（10至10 μg mL）方面表现出良好的线性关系，在超纯水中的检测限为9.7×10 μg mL。使用所开发的方法对添加了浓度为0.005、0.1、1和10 μg mL氧氟沙星的空白蜂蜜、牛奶和猪肉样品进行萃取和测定，回收率在93.1%至105.6%之间。结果支持了该方法强大的应用前景，表明其操作简单、省时且准确性高。
