University of Lyon, Institute of Analytical Sciences, UMR-CNRS 5280, 5, La Doua Street, 69100 Villeurbanne, France; Analytical Chemistry Department, Faculty of Pharmacy, Iuliu Haţieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania.
University of Lyon, Institute of Analytical Sciences, UMR-CNRS 5280, 5, La Doua Street, 69100 Villeurbanne, France.
Talanta. 2015 Jun 1;138:71-76. doi: 10.1016/j.talanta.2015.01.013. Epub 2015 Feb 9.
A simple and highly sensitive approach for the detection of the anti-neoplastic drug gemcitabine is presented, based on a one-step electropolymerized molecularly imprinted microporous-metal-organic-framework. The sensitive layer was prepared by electropolymerization of the aniline moieties of p-aminothiophenol- gold nanoparticles on the surface of gold electrodes tethered with p-aminothiophenol, in the presence of gemcitabine as a template molecule. Experimental parameters that control the performance of the sensor were investigated and optimized. Under optimal conditions a calibration curve was obtained in the linear range from 3.8 fM to 38 nM with a limit of detection of 3 fM. The obtained imprinted sensor has the advantages of easy manufacture, high sensitivity and selectivity and good reproducibility. Furthermore the feasibility of the proposed technique has been investigated on spiked serum samples and infusion solution containing gemcitabine.
提出了一种基于一步电聚合分子印迹微多孔金属有机骨架的检测抗肿瘤药物吉西他滨的简单、高灵敏方法。在金电极表面上，通过电聚合 p-氨基苯硫酚-金纳米粒子的苯胺部分，在以吉西他滨为模板分子的存在下，制备了敏感层。研究并优化了控制传感器性能的实验参数。在最佳条件下，从 3.8 fM 到 38 nM 获得了线性范围的校准曲线，检测限为 3 fM。所得印迹传感器具有制造简单、灵敏度和选择性高、重现性好等优点。此外，还研究了该技术在含有吉西他滨的加标血清样品和输注溶液中的可行性。
