Rus Iulia, Pusta Alexandra, Tertiș Mihaela, Barbălată Cristina, Tomuță Ioan, Săndulescu Robert, Cristea Cecilia
Department of Analytical Chemistry, Iuliu Hațieganu University of Medicine and Pharmacy, 4 Louis Pasteur Street, 400349 Cluj-Napoca, Romania.
Department of Medical Devices, Iuliu Hațieganu University of Medicine and Pharmacy, 4 Louis Pasteur Street, 400349 Cluj-Napoca, Romania.
Pharmaceuticals (Basel). 2021 Sep 10;14(9):912. doi: 10.3390/ph14090912.
The development of fast and easy-to-use methods for gemcitabine detection is of great interest for pharmaceutical formulation control in both research laboratories and hospitals. In this study, we report a simple, fast and direct electrochemical method for gemcitabine detection using a boron-doped diamond electrode. The electrochemical oxidation of gemcitabine on a boron-doped diamond electrode was found to be irreversible in differential pulse voltammetry, and scan rate influence studies demonstrated that the process is diffusion-controlled. The influence of the pH and supporting electrolytes were also tested, and the optimized differential pulse voltammetry method was linear in the range of 2.5-50 μg/mL, with a detection limit of 0.85 μg/mL in phosphate-buffered saline (pH 7.4; 0.1 M). An amperometric method was also optimized for gemcitabine detection. The linear range of the method was 0.5-65 μg/mL in phosphate-buffered saline of pH 7.4 as well as pH 5.5, the limit of detection being 0.15 μg/mL. The optimized differential pulse voltammetry and amperometric detection strategies were successfully applied to pharmaceutical formulations, and the results were compared to those obtained by high-performance liquid chromatography and UV-Vis spectrophotometry with good correlations.
开发快速且易于使用的吉西他滨检测方法，对于研究实验室和医院的药物制剂控制而言都具有重大意义。在本研究中，我们报告了一种使用硼掺杂金刚石电极检测吉西他滨的简单、快速且直接的电化学方法。发现在差分脉冲伏安法中，吉西他滨在硼掺杂金刚石电极上的电化学氧化是不可逆的，扫描速率影响研究表明该过程受扩散控制。还测试了pH值和支持电解质的影响，优化后的差分脉冲伏安法在2.5 - 50μg/mL范围内呈线性，在磷酸盐缓冲盐水（pH 7.4；0.1M）中的检测限为0.85μg/mL。还对用于吉西他滨检测的安培法进行了优化。该方法在pH 7.4以及pH 5.5的磷酸盐缓冲盐水中的线性范围为0.5 - 65μg/mL，检测限为0.15μg/mL。优化后的差分脉冲伏安法和安培检测策略成功应用于药物制剂，并将结果与通过高效液相色谱法和紫外可见分光光度法获得的结果进行比较，具有良好的相关性。
