School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, China.
School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, China.
Talanta. 2021 Aug 15;231:122334. doi: 10.1016/j.talanta.2021.122334. Epub 2021 Mar 27.
A composite fluorescent nanoprobe based on metal enhanced fluorescence (MEF) effect of gelatin-coated silver nanoparticles (AgNPs@gel) was developed for selective and sensitive detection of dopamine (DA). The characteristic fluorescence of Tb(III) was using as the detection signal and AgNPs@gel served as substrates of the MEF. Gelatin with rich amine and carboxylic groups was used not only as a co-ligand of Tb(III) complex, but also as a bridging substance and a spacing material for improving the MEF of AgNPs@gel on the intrinsic luminescent intensity of Tb(III). Under the optimal conditions, the increment of the fluorescence intensity (measured at 307/544 nm as excitation/emission wavelength) of the system increased linearly with the concentration of DA in the range of 0.80-100 nM (R = 0.9937) and 100-1000 nM (R = 0.9978). The fluorescent probe greatly improved Tb(III) luminescence, which paved the way for sensitive detection with a low detection limit of 0.54 nM. It also showed good selectivity among other neurotransmitters. This work was successfully applied to the determination of DA in human serum samples with recoveries ranging from 99.8 to 102.2%. We believe that the Tb(III)-DA-AgNPs@gel composite fluorescent probe can be developed as a new approach for DA detection.
基于明胶包覆的银纳米粒子（AgNPs@gel）的金属增强荧光（MEF）效应，开发了一种复合荧光探针，用于选择性和灵敏检测多巴胺（DA）。Tb(III) 的特征荧光被用作检测信号，AgNPs@gel 用作 MEF 的基底。具有丰富胺基和羧基的明胶不仅可用作 Tb(III) 配合物的共配体，还可用作提高 AgNPs@gel 的 MEF 以及增强 Tb(III) 本征发光强度的桥接物质和间隔材料。在最佳条件下，该体系的荧光强度（在 307/544nm 处作为激发/发射波长测量）的增量与 DA 的浓度在 0.80-100nM（R=0.9937）和 100-1000nM（R=0.9978）范围内呈线性关系。荧光探针大大提高了 Tb(III) 的发光强度，为具有 0.54nM 低检测限的灵敏检测铺平了道路。它在其他神经递质中也表现出良好的选择性。该工作成功地应用于人血清样品中 DA 的测定，回收率在 99.8%至 102.2%之间。我们相信，Tb(III)-DA-AgNPs@gel 复合荧光探针可以作为 DA 检测的一种新方法得到发展。
